Yamamoto K, Tanaka R, Yoshida S, Ono K, Mori H, Taniguchi Y, Oda T, Watanabe T
Department of Neurosurgery, Niigata University, Japan.
J Immunother. 1999 Jan;22(1):33-40. doi: 10.1097/00002371-199901000-00005.
We studied the effect of a streptococcal preparation, OK-432, on the cytotoxicity and the proliferation of lymphokine-activated killer (LAK) cells for use in adoptive immunotherapy. Peripheral blood mononuclear cells (PBMC) were derived from healthy donors and patients with malignant brain tumors. We divided PBMC into two groups; these cells then were stimulated with interleukin-2 in the presence or absence of OK-432. OK-432 was added only in the initial 3 days during the 3-week midterm culture period. Then, we compared OK-432-stimulated LAK (OK-LAK) cells with standard LAK (sLAK) cells in terms of their rate of proliferation and cytotoxicity. OK-LAK cells proliferated more rapidly than sLAK cells. The cytotoxicity of OK-LAK cells increased, whereas that of sLAK cells decreased. We also investigated the phenotypic differences between these two types of LAK cells and found that, on day 21, the OK-LAK cells consisted mostly of CD3-CD56+ NK cells, whereas the sLAK cells consisted mostly of CD3+CD56- T cells. The difference in their level of cytotoxic potential might be explained by the difference of predominant phenotype.
我们研究了一种链球菌制剂OK-432对用于过继性免疫治疗的淋巴因子激活的杀伤（LAK）细胞的细胞毒性和增殖的影响。外周血单个核细胞（PBMC）取自健康供者和恶性脑肿瘤患者。我们将PBMC分为两组；然后在有或无OK-432的情况下用白细胞介素-2刺激这些细胞。在为期3周的中期培养期内，仅在最初3天添加OK-432。然后，我们比较了OK-432刺激的LAK（OK-LAK）细胞和标准LAK（sLAK）细胞的增殖率和细胞毒性。OK-LAK细胞比sLAK细胞增殖更快。OK-LAK细胞的细胞毒性增加，而sLAK细胞的细胞毒性降低。我们还研究了这两种LAK细胞之间的表型差异，发现在第21天，OK-LAK细胞主要由CD3-CD56+自然杀伤（NK）细胞组成，而sLAK细胞主要由CD3+CD56-T细胞组成。它们细胞毒性潜能水平的差异可能由主要表型的差异来解释。
