铜绿假单胞菌新型多药外排泵MexXY在大肠杆菌中的表达
Expression in Escherichia coli of a new multidrug efflux pump, MexXY, from Pseudomonas aeruginosa.
作者信息
Mine T, Morita Y, Kataoka A, Mizushima T, Tsuchiya T
机构信息
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama, Japan.
出版信息
Antimicrob Agents Chemother. 1999 Feb;43(2):415-7. doi: 10.1128/AAC.43.2.415.
Abstract
Two new genes (mexXY) similar to mexAB, mexCD, and mexEF and mediating multidrug resistance were cloned from the chromosome of Pseudomonas aeruginosa. Elevated ethidium extrusion was observed with Escherichia coli cells harboring the plasmid carrying mexXY. This MexXY system confers higher resistance to fluoroquinolones than the MexAB and MexCD systems, and E. coli ToIC or P. aeruginosa OprM is necessary for the function of the MexXY system.
摘要
从铜绿假单胞菌染色体中克隆出了两个与mexAB、mexCD和mexEF相似且介导多药耐药性的新基因（mexXY）。在携带含有mexXY质粒的大肠杆菌细胞中观察到溴化乙锭外排增加。与MexAB和MexCD系统相比，MexXY系统对氟喹诺酮类药物具有更高的耐药性，并且大肠杆菌的ToIC或铜绿假单胞菌的OprM是MexXY系统发挥功能所必需的。
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