Rökaeus A, Jiang K, Spyrou G, Waschek J A
Department of Medical Biochemistry and Biophysics (MBB), Karolinska Institutet, Stockholm, Sweden.
Ann N Y Acad Sci. 1998 Dec 21;863:1-13. doi: 10.1111/j.1749-6632.1998.tb10679.x.
Galanin is a neuropeptide widely expressed in the central and peripheral nervous system where it acts as a neurotransmitter/neuromodulator and possibly an immunoregulator and growth factor. Galanin gene expression is highly regulated during development and by certain hormones and injury situations. We have examined transcriptional control mechanisms for this gene using chimeric bovine galanin/luciferase reporter genes. These were analyzed in cultured cells and in transgenic mice. The studies reveal that enhancer and silencer sequences are involved in conferring cell- and tissue-specific expression, and that specific elements close to the promoter are responsible for nerve growth factor and protein kinase C induction. So far, the studies have not revealed sequences on the bovine gene that mediate the action of estrogen.
甘丙肽是一种神经肽，在中枢和外周神经系统中广泛表达，在这些部位它作为神经递质/神经调质发挥作用，还可能作为免疫调节剂和生长因子。甘丙肽基因表达在发育过程中以及受某些激素和损伤情况的影响下受到高度调控。我们使用嵌合牛甘丙肽/荧光素酶报告基因研究了该基因的转录控制机制。这些基因在培养细胞和转基因小鼠中进行了分析。研究表明，增强子和沉默子序列参与赋予细胞和组织特异性表达，并且启动子附近的特定元件负责神经生长因子和蛋白激酶C的诱导。到目前为止，这些研究尚未揭示牛基因上介导雌激素作用的序列。
