[利用硅铬黄CX - 3B从蜂毒中纯化磷脂酶A2]
[Purification of phospholipase A2 from bee venom on silochrome CX- 3B].
作者信息
Ershov A V, Muzalevskiĭ A I, Ershova O A
机构信息
Omutninsk Chemical Plant, Kirov Region.
出版信息
Prikl Biokhim Mikrobiol. 1998 Nov-Dec;34(6):628-31.
PMID:9929892
Abstract
A new and simple procedure is proposed for the purification of phospholipase A2 from Bee Venom, which includes three stages of chromatography on silochrom CX-3B. We offer original method of desalting and concentration of the enzyme used chromatography on silochrom CX-3B. The activity of enzyme is approx. 1200 U/mg lyophilizate (approx. 1400 U/mg protein).
摘要
提出了一种从蜂毒中纯化磷脂酶A2的新的简单方法，该方法包括在硅色层CX-3B上进行三个阶段的色谱分离。我们提供了使用硅色层CX-3B色谱法对酶进行脱盐和浓缩的原始方法。该酶的活性约为1200 U/mg冻干物（约1400 U/mg蛋白质）。
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