Majde J A, Guha-Thakurta N, Chen Z, Bredow S, Krueger J M
Office of Naval Research, Arlington, Virginia, USA.
Arch Virol. 1998;143(12):2371-80. doi: 10.1007/s007050050467.
The viral factor responsible for triggering the acute phase response, or 'flu' syndrome, associated with many acute viral infections is not defined. One candidate viral factor is double-stranded RNA (dsRNA) generated during viral replication. In this report we demonstrate by reverse-transcriptase polymerase-chain reaction that nuclease-stable viral RNA was released from influenza-infected MDCK epithelial cells at the time of cell lysis. Removal of virion-associated RNA by ultracentrifugation left equal amounts of positive- and negative-strand viral RNA in the medium that resisted degradation by endogenous RNase in the medium and by exogenous RNase added prior to phenol extraction. These data are the first demonstration that viral RNA with characteristics of dsRNA is spontaneously released from dying influenza virus-infected cells, and thus is available to amplify cytokine induction and contribute to systemic disease.
引发与许多急性病毒感染相关的急性期反应或“流感”综合征的病毒因子尚未明确。一种候选病毒因子是病毒复制过程中产生的双链RNA（dsRNA）。在本报告中，我们通过逆转录聚合酶链反应证明，在细胞裂解时，核酸酶稳定的病毒RNA从感染流感病毒的MDCK上皮细胞中释放出来。通过超速离心去除与病毒粒子相关的RNA后，培养基中留下了等量的正链和负链病毒RNA，它们能抵抗培养基中的内源性RNase以及在酚提取之前添加的外源性RNase的降解。这些数据首次证明，具有dsRNA特征的病毒RNA会从垂死的感染流感病毒的细胞中自发释放出来，因此可用于放大细胞因子诱导并导致全身性疾病。
