Coveñas R, de León M, Narváez J A, Aguirre J A, Tramu G, González-Barón S
Department of Cellular Biology and Pathology, University of Salamanca, Spain.
Anat Embryol (Berl). 1999 Feb;199(2):161-7. doi: 10.1007/s004290050217.
Using an indirect immunoperoxidase technique, we studied the location of beta-endorphin (1-27) fibres and cell bodies in the cat brainstem. The highest density of immunoreactive fibres was found in the lateral and medial parabrachial nuclei and in the locus coeruleus; a moderate density was observed in the periaqueductal gray and the central reticular nucleus, and a low density was observed in the interpeduncular nucleus, the nucleus incertus, the raphe pallidus nucleus, the paralemniscal reticular nucleus, the laterodorsal tegmental nucleus, the pericentral division of the dorsal tegmental nucleus and the lateral reticular nucleus. Immunoreactive neurons were observed in the superior central nucleus, the pericentral division of the dorsal tegmental nucleus, the interpeduncular nucleus, the nucleus incertus and the dorsal raphe nucleus. Our results point to a more widespread distribution of beta-endorphin (1-27)-immunoreactive perikarya in the cat brainstem in comparison with previous studies carried out in the same region of other mammals. The distribution of beta-endorphin (1-27)-immunoreactive fibres and perikarya is compared with the location of other neuropeptides in the cat brainstem. Moreover, our findings reveal that beta-endorphin (1-27)-immunoreactive structures are widely distributed in the cat brainstem, suggesting that the peptide might be involved in several physiological functions.
我们采用间接免疫过氧化物酶技术，研究了猫脑干中β-内啡肽（1-27）纤维和细胞体的位置。免疫反应性纤维密度最高的区域位于外侧和内侧臂旁核以及蓝斑；中脑导水管周围灰质和中央网状核的免疫反应性纤维密度中等；脚间核、不确定核、中缝苍白核、旁臂旁网状核、外侧背盖核、背盖核中央周围亚核和外侧网状核的免疫反应性纤维密度较低。在中央上核、背盖核中央周围亚核、脚间核、不确定核和中缝背核中观察到了免疫反应性神经元。与先前在其他哺乳动物同一区域进行的研究相比，我们的结果表明，猫脑干中β-内啡肽（1-27）免疫反应性核周体的分布更为广泛。将β-内啡肽（1-27）免疫反应性纤维和核周体的分布与猫脑干中其他神经肽的位置进行了比较。此外，我们的研究结果表明，β-内啡肽（1-27）免疫反应性结构广泛分布于猫脑干，提示该肽可能参与多种生理功能。
