Panta P, Nanulescu M V, Culea M, Palibroda N
Clinica Pediatrie III, C.M.S.S.C., Cluj Napoca.
Pneumoftiziologia. 1998 Jan-Mar;47(1):15-9.
Long-term therapy with slow-release theophylline is compulsory to be monitorized by measuring serum concentration of the drug. This is due to the narrow therapeutically index of theophylline for monitoring the long term therapy with this drug, and to establish the correlation between the serum and saliva concentration of theophylline. The study group was represented by 13 children with asthma, with the age between 2 and 13 years that received theophylline in an average dosage of 15.2 mg/kg/24 hours. In all of them there were obtained two paired simultaneous samples of venous blood and of saliva, at 4 hours and at 8 hours respectively, after the administration of theophylline. For facilitate the saliva sampling, its production was stimulated by applying citric acid on the tongue and aspiration with a sterile syringe. For measuring the serum and saliva levels of the drug it was used mass spectrometry with 15N-theophylline as internal standard. Serum theophylline concentration varied between 6.56 +/- 4.48 micrograms/ml at 8 hours after drug administration and respectively 7.45 +/- 3.94 micrograms/ml at 4 hours after drug administration. Saliva concentrations of theophylline varied between 4.18 +/- 2.40 micrograms/ml at 8 hours after drug administration and respectively 4.66 +/- 2.39 micrograms/ml at 4 hours after drug administration. The average ratio between serum and saliva concentration in the 24 paired samples was 1.62 +/- 0.18. The intraindividual variation of this ratio was 8.64%.
缓释茶碱的长期治疗必须通过测量药物的血清浓度来进行监测。这是因为茶碱的治疗指数较窄，需要监测该药物的长期治疗情况，并建立茶碱血清浓度与唾液浓度之间的相关性。研究组由13名哮喘儿童组成，年龄在2至13岁之间，平均剂量为15.2毫克/千克/24小时服用茶碱。在所有儿童中，分别在服用茶碱后4小时和8小时同时采集两份配对的静脉血和唾液样本。为便于采集唾液样本，通过在舌头上涂抹柠檬酸并用无菌注射器抽吸来刺激唾液分泌。使用以15N - 茶碱为内标物的质谱法测量药物的血清和唾液水平。给药后8小时血清茶碱浓度在6.56±4.48微克/毫升之间，给药后4小时分别为7.45±3.94微克/毫升。给药后8小时茶碱唾液浓度在4.18±2.40微克/毫升之间，给药后4小时分别为4.66±2.39微克/毫升。24对样本中血清与唾液浓度的平均比值为1.62±0.18。该比值的个体内变异为8.64%。
