Yörükoglu K, Sagol O, Ozkara E, Mungan U, Kirkali Z
Department of Pathology, Dokuz Eylül University, School of Medicine, Izmir, Turkey.
Eur Urol. 1999 Feb;35(2):109-12. doi: 10.1159/000019828.
Quantitation of microvessel density has been shown to predict tumor aggressiveness in prostate carcinoma (Pca). The correlation of estimated values of angiogenesis in needle biopsy and radical prostatectomy materials might show the efficiency of the diagnostic biopsy material to represent a good sample of the entire tumor. In this study, we assessed the correlation of microvascularization computed by morphometric methods in biopsy and radical operation materials.
Sections from diagnostic biopsy and radical prostatectomy materials of 23 patients with Pca were stained with factor- 8-associated antigen by immunohistochemistry and vascular surface density (VSD), microvessel numbers (NVES) and maximum microvessel number (NVES-MAX) were assessed by stereological methods. The microvascularization values in biopsy and radical operation materials were compared by Wilcoxon signed-ranks test for paired samples.
No significant difference was observed between VSD and NVES values that were obtained from biopsy and radical operation materials of the same cases (p = 0.2478 and 0.3458, respectively). However, a significant difference was noted for NVES- MAX (p = 0.0004).
We have shown, in a limited number of cases, that microvascularity prediction by stereological analysis using VSD and NVES in diagnostic needle biopsies correlates well with the radical operation specimens. Preoperative biopsies may predict overall tumor neovascularization characteristics that may have an impact on pathological stage and behavior.
微血管密度定量已被证明可预测前列腺癌（Pca）的肿瘤侵袭性。穿刺活检与根治性前列腺切除标本中血管生成估计值的相关性，可能显示诊断性活检材料作为整个肿瘤良好样本的有效性。在本研究中，我们评估了活检和根治性手术材料中通过形态学方法计算的微血管化的相关性。
对23例Pca患者的诊断性活检和根治性前列腺切除标本进行切片，采用免疫组织化学方法用因子VIII相关抗原染色，并通过体视学方法评估血管表面密度（VSD）、微血管数量（NVES）和最大微血管数量（NVES-MAX）。对配对样本采用Wilcoxon符号秩检验比较活检和根治性手术材料中的微血管化值。
同一病例的活检和根治性手术材料所获得的VSD和NVES值之间未观察到显著差异（分别为p = 0.2478和0.3458）。然而，NVES-MAX有显著差异（p = 0.0004）。
在有限数量的病例中，我们表明在诊断性穿刺活检中使用VSD和NVES通过体视学分析预测微血管与根治性手术标本相关性良好。术前活检可能预测对病理分期和行为有影响的总体肿瘤新生血管特征。
