Biedenbach D J, Beach M L, Jones R N
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA.
Diagn Microbiol Infect Dis. 1998 Dec;32(4):307-11. doi: 10.1016/s0732-8893(98)00114-x.
Gatifloxacin, a new 8-methoxy fluoroquinolone, was tested against 131 strains of Neisseria gonorrhoeae by reference agar dilution, disk diffusion, and Etest (AB BIODISK, Solna, Sweden) methods on supplemented GC agar. Gatifloxacin activity was equal to ciprofloxacin (MIC50, 0.008 microgram/mL) against strains fully susceptible to fluoroquinolones, but was generally four-fold more active (MIC90, 0.064-0.094 microgram/mL) against strains with par C or gyr A mutations and resistance to ciprofloxacin. Etest results were comparable to those generated by the agar dilution test [correlation coefficient (r) = 0.97]. Gatifloxacin zone diameters using 5-microgram disks also correlated well (r = 0.86-0.87) with the agar dilution and Etest MIC results. Breakpoints for laboratory testing of Neisseria gonorrhoeae strains await clinical trial outcome correlations, but susceptibility at < or = 0.125 or < or = 0.25 microgram/mL (> or = 34 mm) seems appropriate. All three tests used in this study seem applicable for laboratory testing of isolates from patients with uncomplicated gonorrhoeae receiving therapy with gatifloxacin.
加替沙星是一种新型8-甲氧基氟喹诺酮类药物，采用参考琼脂稀释法、纸片扩散法及Etest法（AB BIODISK公司，瑞典索尔纳），在添加营养成分的GC琼脂上，对131株淋病奈瑟菌进行了检测。加替沙星对氟喹诺酮类药物完全敏感的菌株的活性与环丙沙星相当（MIC50为0.008微克/毫升），但对携带par C或gyr A突变且对环丙沙星耐药的菌株，其活性通常高4倍（MIC90为0.064 - 0.094微克/毫升）。Etest结果与琼脂稀释试验结果具有可比性[相关系数（r）= 0.97]。使用5微克纸片测得的加替沙星抑菌圈直径与琼脂稀释法和Etest法测得的MIC结果也具有良好的相关性（r = 0.86 - 0.87）。淋病奈瑟菌菌株实验室检测的断点有待临床试验结果的相关性分析，但<或= 0.125或<或= 0.25微克/毫升（>或= 34毫米）时的敏感性似乎是合适的。本研究中使用的所有三种检测方法似乎都适用于对接受加替沙星治疗的单纯性淋病患者分离株进行实验室检测。
