González-Vela M C, Garijo M F, Fernández F, Buelta L, Val-Bernal J F
Anatomical Pathology Department, Marqués de Valdecilla University Hospital, Medical Faculty, University of Cantabria, Santander, Spain.
Histopathology. 1999 Jan;34(1):35-42. doi: 10.1046/j.1365-2559.1999.00548.x.
To determinate the relationship between tumoral angiogenesis and cathepsin D (CD) expression in tumour and host stromal cells of invasive breast carcinoma, and to examine its association with classical prognostic factors such as lymph node status, histological grade, tumour size, mitotic rate, peritumoral lymphovascular invasion and oestrogen receptor (ER) status.
Sections from 102 invasive breast carcinoma were cut from the archival formalin-fixed, paraffin-embedded tissue blocks and stained using immunohistochemistry for the endothelial cell adhesion molecule (CD31) and CD. Microvessel density was assessed by counting vessels in the three most vascular areas at x400 field. The counts were expressed as the highest counts within any x400 field. The evaluation of immunostaining for CD was performed separately in both the parenchymal and stromal cells. Microvessel density was correlated positively with histological grade and peritumoral lymphovascular invasion, and correlated inversely with ER status. Positive CD staining of tumour cells was more frequent in positive ER tumours and was not significantly associated with the other classical prognostic factors. However, moderate to strong staining of host cells was correlated with higher histological grade, higher mitotic index and lack of ER protein. There was a statistically significant association between CD expression of host stromal cells and higher vessel count.
CD in host stromal cells is associated with more aggressive tumours and with a higher intratumoral microvessel density. Evaluation of CD in combination with angiogenic activity may be of some help in more accurately predicting the biological behaviour of breast carcinoma.
确定浸润性乳腺癌肿瘤及宿主基质细胞中肿瘤血管生成与组织蛋白酶D（CD）表达之间的关系，并研究其与经典预后因素如淋巴结状态、组织学分级、肿瘤大小、有丝分裂率、肿瘤周围淋巴管浸润及雌激素受体（ER）状态的关联。
从存档的福尔马林固定、石蜡包埋组织块中切取102例浸润性乳腺癌切片，采用免疫组织化学法对内皮细胞黏附分子（CD31）和CD进行染色。通过在400倍视野下计数三个血管最丰富区域的血管来评估微血管密度。计数结果表示为任何400倍视野内的最高计数。对CD的免疫染色评估分别在实质细胞和基质细胞中进行。微血管密度与组织学分级和肿瘤周围淋巴管浸润呈正相关，与ER状态呈负相关。ER阳性肿瘤中肿瘤细胞CD染色阳性更为常见，且与其他经典预后因素无显著关联。然而，宿主细胞中度至强染色与较高的组织学分级、较高的有丝分裂指数及ER蛋白缺失相关。宿主基质细胞的CD表达与较高的血管计数之间存在统计学上的显著关联。
宿主基质细胞中的CD与更具侵袭性的肿瘤及更高的肿瘤内微血管密度相关。联合评估CD与血管生成活性可能有助于更准确地预测乳腺癌的生物学行为。
