用计算机图像分析仪分析组织蛋白酶D、增殖细胞核抗原和表皮生长因子受体在人乳腺癌中的免疫组织化学定位：与组织学分级和转移行为的相关性

Immunohistochemical localization of cathepsin D, proliferating cell nuclear antigen and epidermal growth factor receptor in human breast carcinoma analysed by computer image analyser: correlation with histological grade and metastatic behaviour.

作者信息

Okamura K, Kobayashi I, Matsuo K, Kiyoshima T, Yamamoto K, Miyoshi A, Sakai H

机构信息

Department of Oral Pathology, Fukuoka Dental College, Kyushu University, Japan.

出版信息

Histopathology. 1997 Dec;31(6):540-8. doi: 10.1046/j.1365-2559.1997.3030894.x.

DOI:10.1046/j.1365-2559.1997.3030894.x

PMID:9447385

Abstract

AIMS

The purpose of this study is to examine the relationship between immunohistochemical localization of cathepsin D (CD), proliferating cell nuclear antigen (PCNA) and epidermal growth factor receptor (EGF-R) in 65 cases of breast carcinoma in Japanese women and traditional prognostic factors such as histological grade, lymph node status, mitotic rate and clinical stage, in order to possibly identify some indicator(s) that may be specifically associated with prognosis.

METHODS AND RESULTS

Serial sections of 5-micron thick were cut from the archival formalin-fixed, paraffin-embedded tissue blocks, and processed for CD, PCNA and EGF-R immunostaining. The results were analysed by computer-based image analysis system. All samples showed a positive immunoreaction for cathepsin D in both the parenchyma and stroma. However, the staining area and intensity varied from cell to cell in the parenchyma and stroma as well as among samples. Subsequently, the evaluation of immunostaining for CD was separately performed in both the parenchyma and stroma (CDpar and CDstr, respectively) and the combination of both components (CDtotal). PCNA and EGF-R showed positive immunostaining almost exclusively in the parenchymal component of the carcinoma tissue specimens. CDtotal significantly correlated with the histological grade, PCNA index (PI), mitotic rate (MR), EGF-R and lymph node metastasis. Significant correlation was also demonstrated between CDpar and the histological grade. EGF-R and lymph node metastasis, or between CDstr and MR, EGF-R and lymph node metastasis. EGF-R correlated highly with the histological grade, MR score, lymph node metastases and recurrence-free survival.

CONCLUSIONS

Both the CD parameters and EGF-R are valuable indicators for predicting the biological behaviour of human breast carcinoma.

摘要

目的

本研究旨在探讨65例日本女性乳腺癌组织中组织蛋白酶D（CD）、增殖细胞核抗原（PCNA）和表皮生长因子受体（EGF-R）的免疫组化定位与组织学分级、淋巴结状态、有丝分裂率及临床分期等传统预后因素之间的关系，以期确定一些可能与预后特异性相关的指标。

方法与结果

从存档的福尔马林固定、石蜡包埋组织块上切取5微米厚的连续切片，进行CD、PCNA和EGF-R免疫染色。结果通过计算机图像分析系统进行分析。所有样本在实质和间质中均显示组织蛋白酶D免疫反应阳性。然而，实质和间质中以及不同样本间的染色面积和强度在细胞间存在差异。随后，分别对实质和间质（分别为CDpar和CDstr）以及两者的组合（CDtotal）进行CD免疫染色评估。PCNA和EGF-R几乎仅在癌组织标本的实质成分中显示阳性免疫染色。CDtotal与组织学分级、PCNA指数（PI）、有丝分裂率（MR）、EGF-R及淋巴结转移显著相关。CDpar与组织学分级、EGF-R及淋巴结转移之间，或CDstr与MR、EGF-R及淋巴结转移之间也显示出显著相关性。EGF-R与组织学分级、MR评分、淋巴结转移及无复发生存率高度相关。