Sameshima S, Sakai K, Nagawa H, Tsuno N, Kitayama J, Muto T
Department of Surgery, University of Tokyo, Japan.
Surg Today. 1999;29(1):55-62. doi: 10.1007/BF02482971.
It has been established that lymphocytes obtained from tumor-draining lymph nodes (DLN) are sensitized to the tumor antigen in vivo. Moreover, after being activated in vitro, these cells can be utilized for adoptive immunotherapy. In the present study, DLN cells, obtained from C57BL/6 mice with fibrosarcoma (MC-1), were activated and expanded with anti-CD3 monoclonal antibody followed by culture with recombinant interleukin-2 (rIL-2). These CD4- CD8+ CD25+ CD44+ T-cells showed specific antitumor efficacy to the pulmonary micrometastases of an autologous tumor, against which lymphokine-activated killer cells were ineffective; however, they did not show cytolytic activity in vitro. The supernatant, obtained by coculturing the activated DLN cells with MC-1 cells, exhibited the specific production of interferon-gamma (IFN-gamma) which was enhanced by rIL-2. The therapeutic effect of the activated DLN cells correlated with the specific IFN-gamma production better than with the cytolytic activity.
已经确定，从肿瘤引流淋巴结（DLN）获得的淋巴细胞在体内对肿瘤抗原敏感。此外，在体外激活后，这些细胞可用于过继性免疫治疗。在本研究中，从患有纤维肉瘤（MC-1）的C57BL/6小鼠获得的DLN细胞，先用抗CD3单克隆抗体激活并扩增，然后用重组白细胞介素-2（rIL-2）培养。这些CD4-CD8+CD25+CD44+T细胞对自体肿瘤的肺微转移显示出特异性抗肿瘤功效，而淋巴因子激活的杀伤细胞对此无效；然而，它们在体外未显示出细胞溶解活性。通过将激活的DLN细胞与MC-1细胞共培养获得的上清液表现出特异性产生γ-干扰素（IFN-γ），rIL-2可增强这种产生。激活的DLN细胞的治疗效果与特异性IFN-γ产生的相关性优于与细胞溶解活性的相关性。
