Chism S E, Wallis S, Burton R C, Warner N L
J Immunol. 1976 Nov;117(5 Pt.2):1870-7.
In previous studies with in vitro activated cytotoxic T lymphocytes, we have demonstrated the presence of oncofetal antigens (OFA) on a range of murine tumor cells. The present studies with the same tumor lines attempt to determine whether these antigens are also capable of activating lymphocyte responses in vivo. Several experimental designs were followed, each being performed many times (1). Preimmunization of mice with irradiated fetal liver cells and followed by challenge with viable tumor cells did not consistently produce a state of tumor resistance. However, injection of live fetal cells frequently led to enhanced tumor growth (2). The growth of tumors in multiparous mice can be inhibited, in contrast to controls, but this effect was relatively short lived after parturition (3). Preimmunization with fetal cells in vivo did not result in augmented secondary cytotoxic T cell responses in vitro (4). Immunization of mice with irradiated tumor cells frequently led to a state of resistance to the clonal growth of hemopoietic fetal cells, although again the level of resistance was usually relatively weak. From the overall results, we conclude that OFA are relatively poor immunogens on tumor cells or fetal cells in vivo, and in contrast to in vitro responses, do not act as potent tumor transplantation antigens.
在先前关于体外激活的细胞毒性T淋巴细胞的研究中，我们已证实在一系列鼠类肿瘤细胞上存在癌胚抗原（OFA）。目前对相同肿瘤细胞系的研究试图确定这些抗原在体内是否也能够激活淋巴细胞反应。采用了几种实验设计，每种设计都进行了多次（1）。用经辐照的胎肝细胞对小鼠进行预免疫，然后用活的肿瘤细胞进行攻击，并未始终产生肿瘤抗性状态。然而，注射活的胎儿细胞常常导致肿瘤生长增强（2）。与对照组相比，经产小鼠体内肿瘤的生长可受到抑制，但这种作用在分娩后持续时间相对较短（3）。在体内用胎儿细胞进行预免疫并未导致体外继发性细胞毒性T细胞反应增强（4）。用经辐照的肿瘤细胞对小鼠进行免疫常常导致对造血胎儿细胞克隆生长的抗性状态，尽管抗性水平通常相对较弱。从总体结果来看，我们得出结论，OFA在体内作为肿瘤细胞或胎儿细胞上的免疫原相对较弱，并且与体外反应相反，不作为有效的肿瘤移植抗原。
