Beggs J D, Cook A M, Fewson C A
J Gen Microbiol. 1976 Oct;96(2):365-74. doi: 10.1099/00221287-96-2-365.
Formation of benzoate and catechol during oxidation of benzyl alcohol by washed suspensions of Acinetobacter calcoaceticus NCIB8250 confirmed earlier results indicating that this organism metabolizes benzyl alcohol via benzaldehyde, benzoate, and the 3-oxoadipate pathway. There was no evidence for feedback inhibition of benzyl alcohol dehydrogenase or benzaldehyde dehydrogenase II. Examination of growth curves and patterns of substrate utilization, as well as measurement of enzyme activities, showed that benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II are repressed when A. calcoaceticus utilizes L-mandelate or phenylglyoxylate. Growth of bacteria on L-mandelate prior to their inoculation into benzyl alcohol/salts medium leads to an exceptionally long lag period before benzyl alcohol is used at the maximum rate. Benzyl alcohol metabolism is also suppressed during growth on benzoate.
用醋酸钙不动杆菌NCIB8250的洗涤悬浮液氧化苯甲醇过程中苯甲酸酯和儿茶酚的形成证实了早期的结果，表明该生物体通过苯甲醛、苯甲酸酯和3-氧代己二酸途径代谢苯甲醇。没有证据表明存在对苯甲醇脱氢酶或苯甲醛脱氢酶II的反馈抑制。对生长曲线和底物利用模式的检查以及酶活性的测量表明，当醋酸钙不动杆菌利用L-扁桃酸或苯乙酮酸时，苯甲醇脱氢酶和苯甲醛脱氢酶II会受到抑制。细菌在接种到苯甲醇/盐培养基之前在L-扁桃酸上生长会导致在以最大速率使用苯甲醇之前出现异常长的延迟期。在苯甲酸酯上生长期间，苯甲醇代谢也会受到抑制。
