Alumets J, Håkanson R, Sundler F, Chang K J
Histochemistry. 1978 Jul 12;56(3-4):187-96. doi: 10.1007/BF00495979.
The distribution and cellular localization of leu-enkephalin in the gut and pancreas was studied by immunohistochemistry using two different antisera, one specifically directed against leu-enkephalin and the other cross reacting with met-enkephalin. The results were identical with both antisera. In all species examined, enkephalin-immunoreactive material was found in nerves of the smooth muscle, particularly numerous in the myenteric plexus. Here, immunoreactive nerve cell bodies were observed occasionally. In addition, enkephalin-immunoreactive material was demonstrated in gut endocrine cells of chicken, mouse, rat, pig and monkey but not of guinea pig, cat and man. Enkephalin cells were detected also in the exocrine parenchyma of the porcine pancreas. They were rare in the gut of mouse, rat and monkey but numerous in the antrum and duodenum of pig where they were identified as 5-hydroxytryptamine-storing enterochromaffin cells. The enkephalin-containing cells of the porcine antrum and duodenum were defined ultrastructurally by the consecutive semithin/ultrathin section technique. The ultrastructural features were typical of enterochromaffin cells, the most characteristic ones being the irregular shape and high electron density of the cytoplasmic granules. The immunoreactive material was confined to the cytoplasmic granules.
利用两种不同的抗血清，通过免疫组织化学方法研究了亮氨酸脑啡肽在肠道和胰腺中的分布及细胞定位。一种抗血清特异性针对亮氨酸脑啡肽，另一种与甲硫氨酸脑啡肽发生交叉反应。两种抗血清得到的结果相同。在所检查的所有物种中，在平滑肌神经中发现了脑啡肽免疫反应性物质，在肌间神经丛中尤其丰富。在此，偶尔观察到免疫反应性神经细胞体。此外，在鸡、小鼠、大鼠、猪和猴的肠道内分泌细胞中证实有脑啡肽免疫反应性物质，但在豚鼠、猫和人的肠道内分泌细胞中未发现。在猪胰腺的外分泌实质中也检测到脑啡肽细胞。它们在小鼠、大鼠和猴的肠道中很少见，但在猪的胃窦和十二指肠中很多，在那里它们被鉴定为储存5-羟色胺的肠嗜铬细胞。通过连续半薄/超薄切片技术，在超微结构上确定了猪胃窦和十二指肠中含脑啡肽的细胞。超微结构特征是肠嗜铬细胞的典型特征，最具特征性的是细胞质颗粒的不规则形状和高电子密度。免疫反应性物质局限于细胞质颗粒中。
