Coronetti E, Lippman M M
J Natl Cancer Inst. 1976 Jun;56(6):1275-7. doi: 10.1093/jnci/56.6.1275.
Suspension cultures of TA3-Ha mouse mammary tumor cells were treated in vitro with a single dose of macromomycin(B) (MCR) at 1 mug/ml for 24 hours. This dose, which is cytostatic but not lethal, increased the immunogenicity of the cells. Adoptive transfer of spleen cells sensitized to MCR/TA3-Ha cells evoked an immune response against MCR-treated TA3-Ha cells but not against normal TA3-Ha cells. The therapeutic effect of MCR treatment (in this case) was, to a very small extent, due to the cytostatic action and more profoundly to the increased immunogenicity of the cells so treated.
将TA3-Ha小鼠乳腺肿瘤细胞的悬浮培养物在体外以1微克/毫升的单剂量大分子霉素（B）（MCR）处理24小时。该剂量具有细胞抑制作用但无致死性，可增强细胞的免疫原性。对MCR/TA3-Ha细胞致敏的脾细胞的过继转移引发了针对经MCR处理的TA3-Ha细胞的免疫反应，但对正常TA3-Ha细胞无反应。在这种情况下，MCR处理的治疗效果在很小程度上归因于细胞抑制作用，而更主要地归因于经如此处理的细胞免疫原性的增强。
