Lin L, Xu B, Rote N S
Department of Microbiology and Immunology, Wright State University School of Medicine, Dayton, Ohio 45435, USA.
Placenta. 1999 Jan;20(1):109-18. doi: 10.1053/plac.1998.0337.
Differentiation-related expression of endogenous retrovirus ERV-3 env in the normal human placental syncytiotrophoblast suggests a role in placental development. The choriocarcinoma cell line BeWo, a model of trophoblast differentiation, is maintained in an undifferentiated state and undergoes differentiation upon the addition of forskolin. The expression of ERV-3 env mRNA increased after 48 h forskolin treatment, concurrently with increased intercellular fusion and production of human chorionic gonadotropin (beta-hCG) mRNA, a hormonal differentiation marker for trophoblast. Over expression of ERV-3 env induced differentiation of BeWo characterized by decreased cell growth, differentiation-related morphologic changes, and induction of beta-hCG mRNA. These results support the first known role for the expression of an endogenous retrovirus in trophoblast differentiation.
内源性逆转录病毒ERV-3 env在正常人类胎盘合体滋养层细胞中的分化相关表达表明其在胎盘发育中发挥作用。绒癌细胞系BeWo是滋养层细胞分化的模型，其处于未分化状态，添加福司可林后会发生分化。福司可林处理48小时后，ERV-3 env mRNA的表达增加，同时细胞间融合增加以及人绒毛膜促性腺激素（β-hCG）mRNA产生增加，β-hCG是滋养层细胞的激素分化标志物。ERV-3 env的过表达诱导了BeWo细胞的分化，其特征为细胞生长减少、出现与分化相关的形态学变化以及β-hCG mRNA的诱导。这些结果支持了内源性逆转录病毒表达在滋养层细胞分化中首次被发现的作用。
