Jacobs W, van Dam G, Bogers J, Deelder A, Van Marck E
Department of Pathology, University of Antwerp (U.I.A.), Antwerpen, Belgium.
Parasitol Res. 1999 Jan;85(1):7-13. doi: 10.1007/s004360050499.
Adult Schistosoma mansoni worms can positively modulate soluble egg antigen (SEA)-induced granulomas formed around SEA-coupled beads implanted in the liver. In this study, our aim was to further unravel the immunopathological characteristics of S.mansoni-worm-derived antigens in vivo. (a) Adult worm antigen (AWA)-coupled Sepharose beads, implanted into the liver, induced granulomas, containing numerous eosinophilic granulocytes and elicited marked periparticular fibrosis (composed of interstitial matrix proteins and basement membrane components). (b) Quantitative morphological analysis demonstrated that in naive mice, AWA-induced hepatic granuloma formation peaked in volume 16 days after injection of the beads. An accelerated response against AWA-coupled particles (peak volume at 8 days) was observed in mice carrying a single-sex, male S. mansoni infection. (c) When the granuloma volume induced by SEA-coupled beads in unisexually S. mansoni infected mice was compared to granulomas induced by beads laden with both SEA and AWA in unsensitized mice, no significant differences in granuloma volume were seen, indicating the existence of in vivo egg/worm antigen cross-sensitization. (d) Naive mice, sensitized with the worm antigens circulating anodic antigen (CAA) or circulating cathodic antigen (CCA), mounted a strongly accelerated response towards SEA-coupled beads implanted in the liver. We infer that, in vivo, worm antigens cross-sensitize with egg antigens and have both granulomogenic and fibrogenic characteristics. The S. mansoni soluble worm antigens CCA and CAA prime hepatic egg-antigen-induced granuloma formation possibly through the presence of immunogenic carbohydrates. These mechanisms lead to an accelerated response against SEA.
曼氏血吸虫成虫可正向调节可溶性虫卵抗原（SEA）诱导的肉芽肿形成，这些肉芽肿围绕植入肝脏的SEA偶联珠形成。在本研究中，我们的目的是进一步阐明曼氏血吸虫成虫来源抗原在体内的免疫病理特征。（a）植入肝脏的成虫抗原（AWA）偶联琼脂糖珠诱导形成肉芽肿，其中含有大量嗜酸性粒细胞，并引发明显的颗粒周围纤维化（由间质基质蛋白和基底膜成分组成）。（b）定量形态学分析表明，在未致敏小鼠中，AWA诱导的肝肉芽肿形成在注射珠子后16天体积达到峰值。在感染单性雄虫曼氏血吸虫的小鼠中观察到对AWA偶联颗粒的加速反应（8天达到峰值体积）。（c）将单性感染曼氏血吸虫小鼠中SEA偶联珠诱导的肉芽肿体积与未致敏小鼠中同时负载SEA和AWA的珠子诱导的肉芽肿体积进行比较，未发现肉芽肿体积有显著差异，表明存在体内虫卵/虫体抗原交叉致敏。（d）用虫体抗原循环阳极抗原（CAA）或循环阴极抗原（CCA）致敏的未致敏小鼠，对植入肝脏的SEA偶联珠产生强烈的加速反应。我们推断，在体内，虫体抗原与虫卵抗原交叉致敏，具有肉芽肿形成和纤维化的特征。曼氏血吸虫可溶性虫体抗原CCA和CAA可能通过免疫原性碳水化合物的存在引发肝虫卵抗原诱导的肉芽肿形成。这些机制导致对SEA的加速反应。
