Robin H, Hoang-Xuan T, Prisant O, Toublanc M, Prost C
Department of Ophthalmology, Hospital Bichat, Paris, France.
Graefes Arch Clin Exp Ophthalmol. 1999 Jan;237(1):39-44. doi: 10.1007/s004170050192.
Immune deposits can be found on the conjunctival basement membrane zone of patients affected by cicatricial pemphigoid using immunofluorescence technique. The purpose of this study was to perform direct immunoelectron microscopy on the conjunctiva of patients with scarring conjunctivitis associated with cicatricial pemphigoid.
Conjunctival and skin biopsies were performed in six patients who presented with presumed autoimmune cicatrizing conjunctivitis associated with cicatricial pemphigoid. Specimens were processed for direct immunofluorescence and direct immunoelectron microscopy.
Direct immunofluorescence was positive in all skin samples and in three of six conjunctival samples. Direct immunoelectron microscopy showed immune deposits in the lamina lucida and the lamina densa of all skin and conjunctival samples.
Direct immunoelectron microscopy can be performed on the conjunctiva. It shows the precise localization of cicatricial pemphigoid target antigens within the conjunctival basement membrane zone.
使用免疫荧光技术可在瘢痕性类天疱疮患者的结膜基底膜区发现免疫沉积物。本研究的目的是对与瘢痕性类天疱疮相关的瘢痕性结膜炎患者的结膜进行直接免疫电子显微镜检查。
对6例表现为疑似与瘢痕性类天疱疮相关的自身免疫性瘢痕性结膜炎患者进行结膜和皮肤活检。标本进行直接免疫荧光和直接免疫电子显微镜检查。
所有皮肤样本和6例结膜样本中的3例直接免疫荧光呈阳性。直接免疫电子显微镜显示所有皮肤和结膜样本的透明板和致密板中有免疫沉积物。
可对结膜进行直接免疫电子显微镜检查。它显示了瘢痕性类天疱疮靶抗原在结膜基底膜区内的精确定位。
