Demers P E, Robin H, Prost C, Toutblanc M, Hoang-Xuan T
Department of Ophthalmology, Bichat-Claude-Bernard Hospital, Paris, France.
Curr Eye Res. 1998 Aug;17(8):823-7.
Optimizing the sensitivity of immunopathologic methods in detecting target antigens in immune-mediated cicatrizing conjunctivitis.
Immunofluorescence was performed on normal and salt-split conjunctival biopsies in fifteen patients with clinical evidence of ocular cicatricial pemphigoid, and results were compared with immunoperoxidase, a technique thought to be more sensitive although more expensive and more difficult technically to perform.
Ten of fifteen biopsies (67%) were positive when conventional and salt-split immunofluorescence results were combined. Four of eight patients with positive conventional immunofluorescence showed more marked immunofluorescence with the salt-split method. All patients were positive with immunoperoxidase (100%).
Immunoperoxidase was more sensitive than conventional or salt-split immunofluorescence in detecting immunoreactant deposition along the basement membrane of the conjunctiva. Salt-split immunofluorescence demonstrated more intense staining of conjunctival samples when compared with conventional immunofluorescence, without however increasing the yield of positive biopsies. Finding solutions for the proper handling of conjunctival tissue in salt may improve the diagnostic yield of salt-split immunofluorescence.
优化免疫病理学方法在检测免疫介导的瘢痕性结膜炎中靶抗原的敏感性。
对15例有眼部瘢痕性类天疱疮临床证据的患者的正常及盐裂结膜活检组织进行免疫荧光检测，并将结果与免疫过氧化物酶法进行比较，免疫过氧化物酶法虽技术上更昂贵、操作更困难，但被认为更敏感。
当传统免疫荧光和盐裂免疫荧光结果相结合时，15例活检组织中有10例（67%）呈阳性。8例传统免疫荧光阳性的患者中有4例在盐裂法检测时显示出更明显的免疫荧光。所有患者免疫过氧化物酶检测均为阳性（100%）。
在检测结膜基底膜处的免疫反应物沉积时，免疫过氧化物酶法比传统或盐裂免疫荧光法更敏感。与传统免疫荧光相比，盐裂免疫荧光显示结膜样本染色更强，但并未提高活检阳性率。找到盐中结膜组织妥善处理的方法可能会提高盐裂免疫荧光的诊断阳性率。
