Schneider A, Plessmann U, Felleisen R, Weber K
University of Fribourg, Institute of Zoology, Switzerland.
Parasitol Res. 1999 Mar;85(3):246-8. doi: 10.1007/s004360050542.
Reverse-transcriptase polymerase chain reaction cloning of the 3'-ends of the alpha-tubulin cDNAs of Tritrichomonas mobilensis in combination with Southern-blot analysis identified seven to eight distinct alpha-tubulin genes. All seven lack a carboxy-terminal tyrosine and the corresponding sequence compatible with posttranslational tyrosination. This indicates that whereas tyrosination of alpha-tubulin has been found in most species, including humans and trypanosomes, it is absent in trichomonads.
通过逆转录酶聚合酶链反应克隆运动三毛滴虫α-微管蛋白cDNA的3'末端，并结合Southern印迹分析，鉴定出七到八个不同的α-微管蛋白基因。所有这七个基因都缺乏羧基末端酪氨酸以及与翻译后酪氨酸化相容的相应序列。这表明，虽然在大多数物种中都发现了α-微管蛋白的酪氨酸化，包括人类和锥虫，但在毛滴虫中却不存在。
