Nakajima M, Kawanami O, Jin E, Ghazizadeh M, Honda M, Asano G, Horiba K, Ferrans V J
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School, Kawasaki, Japan.
Pathol Int. 1998 Dec;48(12):944-53. doi: 10.1111/j.1440-1827.1998.tb03865.x.
Immunohistochemical studies were made of the distribution of various cytokeratins (CK), Clara cell secretory protein (CC10), surfactant protein A (SP-A) and type VII collagen in normal human airways. Electron microscopic studies were made to identify hemidesmosomes and anchoring fibrils on the basal surfaces of the epithelial cells. CK19 was detected in all epithelial cells, and CK17 in all basal cells. CK14 was coexpressed in a few basal cells, and this coexpression was decreased in the distal airways. Two types of basal cells were recognized. One type, found mainly in large airways, was characterized by abundant intermediate filaments and well-developed hemidesmosomes and anchoring fibrils. The second type contained few intermediate filaments and poorly developed hemidesmosomes and anchoring fibrils. Reactivity for type VII collagen was found along the basement membrane throughout the airways, but not in the alveoli. Clara cells were reactive for CC10 and CK17, but not for CK14 and SP-A. The bronchiolar cuboidal cells in the respiratory bronchioles were positive only for CK19. Surfactant protein A was present only in type II alveolar epithelial cells. Thus, two types of basal cells are present in airways, and the bronchiolar cuboidal cells appear distinct from these basal cells, Clara cells and type II alveolar epithelial cells.
对正常人气道中各种细胞角蛋白（CK）、克拉拉细胞分泌蛋白（CC10）、表面活性蛋白A（SP-A）和VII型胶原的分布进行了免疫组织化学研究。进行电子显微镜研究以识别上皮细胞基底面的半桥粒和锚定原纤维。在所有上皮细胞中均检测到CK19，在所有基底细胞中均检测到CK17。CK14在少数基底细胞中共表达，且这种共表达在远端气道中减少。识别出两种类型的基底细胞。一种主要存在于大气道中，其特征是中间丝丰富，半桥粒和锚定原纤维发育良好。第二种类型的中间丝较少，半桥粒和锚定原纤维发育不良。在整个气道的基底膜上均发现了VII型胶原的反应性，但在肺泡中未发现。克拉拉细胞对CC10和CK17有反应，但对CK14和SP-A无反应。呼吸性细支气管中的细支气管立方上皮细胞仅对CK19呈阳性。表面活性蛋白A仅存在于II型肺泡上皮细胞中。因此，气道中存在两种类型的基底细胞，细支气管立方上皮细胞与这些基底细胞、克拉拉细胞和II型肺泡上皮细胞不同。
