（2'-5'）和（3'-5'）磷酸二酯键对水溶液中胞苷酰-胞苷的构象和堆积性质的影响。
The effect of (2'-5') and (3'-5') phosphodiester linkages on conformational and stacking properties of cytidylyl-cytidine in aqueous solution.
作者信息
Ezra F S, Kondo N S, Ainsworth C F, Danyluk S S
出版信息
Nucleic Acids Res. 1976 Oct;3(10):2549-62. doi: 10.1093/nar/3.10.2549.
Abstract
Conformational properties of (2'-5') and (3'-5') CpC have been determined by proton magnetic resonance spectroscopy at 220 MHz. The ribose ring structures are predominantly 3E with the exception of the ring from the 2'-phosphate fragment of C(2'-5')pC which exhibits an 2E pucker. Bases are oriented anti with respect to the ribose and the conformations about C4'-C5', C5'-O5', C3'-O3' (C2'-O2') are gg, g'g', and g+ in equilibrium g-, respectively. The dimers exist as mixtures of stacked (g+g+ and g-g- about the P-O(C) bonds) and unstacked species at 20 degrees C. Stacking is estimated to be 35% in both dimers.
摘要
已通过220兆赫的质子磁共振光谱法测定了（2'-5'）和（3'-5'）CpC的构象性质。除了来自C（2'-5'）pC的2'-磷酸片段的环呈现2E褶皱外，核糖环结构主要为3E。碱基相对于核糖呈反式取向，并且关于C4'-C5'、C5'-O5'、C3'-O3'（C2'-O2'）的构象分别为gg、g'g'和g+处于g-平衡态。在20摄氏度时，二聚体以堆叠态（围绕P-O（C）键为g+g+和g-g-）和未堆叠态的混合物形式存在。两种二聚体中的堆叠估计均为35%。
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