Frank M, Akera T, Brody T M
Res Commun Chem Pathol Pharmacol. 1976 Nov;15(3):511-23.
The relationship between cellular calcium movements and contractility was indirectly assessed in Langendorff-preparations of guinea pig hearts perfused with either Krebs-Henseleit solution or a high-potassium (22 mM) solution containing 2.4 X 10(-8) M isoproterenol. The addition of either D600 (2.4-24.0 X 10(-8) M) or ryanodine (0.24-24.0 X 10(-6) M) to hearts perfused with Krebs-Hanseleit solution produced a concentration-dependent reduction in contractile force. In potassium-depolarized, isoproterenol-restored hearts, the negative inotropic action of D600 exhibited concentration-and time-dependent changes similar to those observed in Krebs-Hanseleit solution. In contrast, addition of 0.24-75.0 X 10(6) M ryanodine to potassium-depolarized, isoproterenol-restored hearts produce a time-dependent reduction in tension followed by a concentration-dependent rebound in contractile force. These results have been interpreted to be a consequence of the respective actions of D600 and ryanodine on Ca2+ influx and on intracellular calcium stores.
在以Krebs-Henseleit溶液或含有2.4×10⁻⁸M异丙肾上腺素的高钾（22 mM）溶液灌注的豚鼠心脏Langendorff标本中，间接评估了细胞钙运动与收缩性之间的关系。向用Krebs-Hanseleit溶液灌注的心脏中添加D600（2.4 - 24.0×10⁻⁸M）或ryanodine（0.24 - 24.0×10⁻⁶M）会导致收缩力呈浓度依赖性降低。在钾去极化、异丙肾上腺素恢复的心脏中，D600的负性肌力作用表现出与在Krebs-Hanseleit溶液中观察到的类似的浓度和时间依赖性变化。相反，向钾去极化、异丙肾上腺素恢复的心脏中添加0.24 - 75.0×10⁻⁶M ryanodine会导致张力随时间降低，随后收缩力出现浓度依赖性反弹。这些结果被解释为D600和ryanodine分别对Ca²⁺内流和细胞内钙储存作用的结果。
