Terao T, Owen C A
Tohoku J Exp Med. 1976 Nov;120(3):209-17. doi: 10.1620/tjem.120.209.
Eleven organs of the rat were studied 1 hr and 24 hr after the intravenous administration of 67CuCl2 and 67Cu-ceruloplasmin. The rats were normal, copper-deficient, or copper-laden. The amounts of stable copper and 67Cu in the whole organ and supernatant fractions, corrected for whole blood copper and 67Cu, were measured. The distribution of supernatant 67Cu was determined in three Sephadex G-100 chromatographic zones: Peak I (150,000 daltons), Peak II (31,000 daltons), and Peak III (11,000 daltons). All organs took up 67Cu from both sources, but there was a tendency for increased uptakes in copper-deficient rats and decreased uptake in copper-laden rats. Only lung, heart, and testis took up more 67Cu from 67Cu-ceruloplasmin than from 67CuCl2. Supernatant 67Cu tended to be in Peak I when the source was 67Cu-ceruloplasmin and in Peak II when the source was 67CuCl2. When 67Cu-ceroloplasmin was added to supernatant fractions in vitro, the 67Cu was in Peak I. However, when 67CuCl2 was added to supernatant fractions, Peak III predominated in kidney, brain, testis, and liver; Peak II predominated in none; and Peak I predominated in spleen, muscle, large and small bowel, stomach, lung, and heart. A high-molecular-weight copper-binding substance seems to be present in organ supernatant fractions.
在静脉注射67CuCl2和67Cu-铜蓝蛋白后1小时和24小时，对大鼠的11个器官进行了研究。大鼠分为正常、缺铜或富铜组。测量了全器官和上清液部分中稳定铜和67Cu的含量，并对全血铜和67Cu进行了校正。在上清液67Cu的三个葡聚糖G-100色谱区（峰I，150,000道尔顿；峰II，31,000道尔顿；峰III，11,000道尔顿）测定了其分布。所有器官都从这两种来源摄取67Cu，但缺铜大鼠的摄取量有增加趋势，富铜大鼠的摄取量有减少趋势。只有肺、心脏和睾丸从67Cu-铜蓝蛋白中摄取的67Cu比从67CuCl2中摄取的更多。当来源为67Cu-铜蓝蛋白时，上清液67Cu倾向于在峰I中；当来源为67CuCl2时，上清液67Cu倾向于在峰II中。当在体外向上清液部分中加入67Cu-铜蓝蛋白时，67Cu在峰I中。然而，当向上清液部分中加入67CuCl2时，峰III在肾脏、大脑、睾丸和肝脏中占主导；在其他器官中峰II均不占主导；峰I在脾脏、肌肉、大肠和小肠、胃、肺和心脏中占主导。器官上清液部分似乎存在一种高分子量的铜结合物质。
