Gallie G J, Sewell M M
Trop Anim Health Prod. 1976 Nov;8(4):233-42.
Three groups of calves aged 6 months were completely protected against oral challenge with Taenia saginata eggs following immunisation by three different methods. These were hyperimmunisation with six serial inoculations of a homogenate of T. saginata strobila in Freund's complete adjuvant, a single intramuscular inoculation with hatched T. saginata eggs or a single oral dose of unhatched T. saginata eggs. The calves immunised with tapeworm homogenate developed the strongest haemagglutinating and precipitating antibody response to the complex of antigens in an extract of tapeworm strobila, cysticercus tissue or cysticercus fluid. The orally infected calves developed a moderate antibody response to these antigens but the calves inoculated with hatched eggs showed only a very weak antibody response. The calves infected orally with eggs developed a peripheral eosinophilia but the other two methods of immunisation did not evoke this response. After challenge infection all groups showed an increase in peripheral eosinophil counts except the group immunised with tapeworm homogenate.
三组6月龄的小牛在采用三种不同方法免疫后，对牛带绦虫卵口服攻击均获得完全保护。这三种方法分别是：用牛带绦虫节片匀浆在弗氏完全佐剂中进行六次连续接种进行超免疫；单次肌内接种孵化出的牛带绦虫卵；或单次口服未孵化的牛带绦虫卵。用绦虫匀浆免疫的小牛对绦虫节片、囊尾蚴组织或囊尾蚴液提取物中的抗原复合物产生了最强的血凝和沉淀抗体反应。口服感染的小牛对这些抗原产生了中等程度的抗体反应，但接种孵化出的卵的小牛仅表现出非常微弱的抗体反应。口服感染卵的小牛出现外周嗜酸性粒细胞增多，但其他两种免疫方法未引发这种反应。攻击感染后，除用绦虫匀浆免疫的组外，所有组的外周嗜酸性粒细胞计数均增加。
