节杆菌属细菌产生的一种硫酸化多糖-肽聚糖复合物的结构研究

Structural study on a sulfated polysaccharide-peptidoglycan complex produced by Arthrobacter sp.

作者信息

Yamazaki K, Suzuki M, Inukai K, Kuga H, Korenaga H

机构信息

Basic Technology Research Laboratory, Daiichi Pharmaceutical Co., Ltd., Tokyo, Japan.

出版信息

Biosci Biotechnol Biochem. 1998 Nov;62(11):2138-44. doi: 10.1271/bbb.62.2138.

DOI:10.1271/bbb.62.2138

PMID:9972234

Abstract

The structure of a sulfated polysaccharide-peptidoglycan complex (SP-PG) produced by Arthrobacter sp. was analyzed by NMR spectroscopy. In addition, oligosaccharide fragments of the SP-PG-L obtained by HF degradation were analyzed by NMR spectroscopy. These findings indicated that the sulfated polysaccharide (SP) contains a repeating unit composed of two galactofuranosides and a glucopyranoside. The main chain of the trisaccharide is [-->6) beta-D-Galf(1-->6)-beta-D-Galf(1-->ln, with beta-D-Glcp linked to one of the Galfs through a (1-->2) linkage. The sulfated positions of the trisaccharide were identified as C-3 and C-5 of the beta-glucosylated Galf residues, and C-2 or C-3 of the other Galf residue.

摘要

对节杆菌属（Arthrobacter sp.）产生的一种硫酸化多糖-肽聚糖复合物（SP-PG）的结构进行了核磁共振光谱分析。此外，通过HF降解获得的SP-PG-L的寡糖片段也进行了核磁共振光谱分析。这些结果表明，硫酸化多糖（SP）包含由两个呋喃半乳糖苷和一个吡喃葡萄糖苷组成的重复单元。三糖的主链为[→6)β-D-呋喃半乳糖(1→6)-β-D-呋喃半乳糖(1→]n，其中β-D-吡喃葡萄糖通过(1→2)键与其中一个呋喃半乳糖相连。三糖的硫酸化位置被确定为β-葡萄糖基化呋喃半乳糖残基的C-3和C-5，以及另一个呋喃半乳糖残基的C-2或C-3。