Miquel M, Aguilar M A, Aragòn C M
Area de Psicobiologia, Universitat Jaume I, Castelló, Spain.
Pharmacol Biochem Behav. 1999 Feb;62(2):361-6. doi: 10.1016/s0091-3057(98)00173-7.
It has been reported that ascorbic acid (AA) antagonizes the physiological and behavioral effects of dopamine (DA). AA reduces locomotor activity induced by dopaminergic agonist drugs. Also, AA amplifies the action of antidopaminergic drugs. Ethanol, like other drugs, produces a release of DA in the mesolimbic pathway, and at some doses, induces locomotor activity in mice. The ethanol-induced locomotor activity could be dopamine-dependent because it can be reduced by antidopaminergic drugs. In the present study, we investigated whether an acute administration of AA reduces ethanol-induced locomotor behavior. AA, at doses (0.0, 21.85, 87.5, 175, 350. and 1400 mg/kg) was injected i.p. into mice, 0, 30, 60, or 90 min before an i.p. injection of ethanol (0.0, 0.8, 1.6, 2.4, and 3.2 g/kg). Locomotor activity was evaluated in open-field chambers. Our results showed that AA (350 and 1400 mg/kg) reduced ethanol-induced locomotor activity when injected 30 min before ethanol treatment. This effect was lost when ethanol was administered 90 min after AA injection. AA also reduced locomotor activity produced by d-amphetamine and methanol. The results support a pro-dopaminergic action of ethanol, and suggest a common dopaminergic pathway for the drugs of abuse in locomotor activity.
据报道，抗坏血酸（AA）可拮抗多巴胺（DA）的生理和行为效应。AA可降低多巴胺能激动剂药物诱导的运动活性。此外，AA可增强抗多巴胺能药物的作用。乙醇与其他药物一样，可使中脑边缘通路中的DA释放，并且在某些剂量下可诱导小鼠的运动活性。乙醇诱导的运动活性可能依赖于多巴胺，因为它可被抗多巴胺能药物降低。在本研究中，我们调查了急性给予AA是否会降低乙醇诱导的运动行为。将AA以不同剂量（0.0、21.85、87.5、175、350和1400mg/kg）腹腔注射到小鼠体内，在腹腔注射乙醇（0.0、0.8、1.6、2.4和3.2g/kg）前0、30、60或90分钟进行注射。在旷场实验箱中评估运动活性。我们的结果表明，在乙醇处理前30分钟注射AA（350和1400mg/kg）可降低乙醇诱导的运动活性。当在注射AA后90分钟给予乙醇时，这种效应消失。AA还可降低由右旋苯丙胺和甲醇产生的运动活性。这些结果支持乙醇的促多巴胺能作用，并提示滥用药物在运动活性方面存在共同的多巴胺能通路。
