Seaman R L, Belt M L, Doyle J M, Mathur S P, Prihoda T J
Department of Radiology, Center for Environmental Radiation Toxicology, The University of Texas Health Science Center at San Antonio, 78284, USA.
Int J Radiat Biol. 1999 Jan;75(1):115-20. doi: 10.1080/095530099140870.
To investigate the extent of genetic damage in the peripheral blood and bone marrow cells of mice exposed to ultra-wideband electromagnetic radiation (UWBR).
CF-1 male mice were exposed to UWBR for 15 min at an estimated whole-body average specific absorption rate of 37 mW x kg(-1). Groups of untreated control and positive control mice injected with mitomycin C were also included in the study. After various treatments, half of the mice were killed at 18 h, and the other half at 24 h. Peripheral blood and bone marrow smears were examined to determine the extent of genotoxicity, as assessed by the presence of micronuclei (MN) in polychromatic erythrocytes (PCE).
The percentages of PCE and the incidence of MN per 2000 PCE in both tissues in mice killed at 18 h were similar to the frequencies observed in mice terminated at 24 h. There were no significant differences in the percentage of PCE between control and the mice with or without UWBR exposure; the group mean values (+/- standard deviation) were in the range of 3.1+/-0.14 to 3.2+/-0.23 in peripheral blood, and 49.0+/-3.56 to 52.3+/-4.02 in bone marrow. The mean incidence of MN per 2000 PCE in control and in mice with or without UWBR exposure ranged from 7.7+/-2.00 to 9.7+/-2.54 in peripheral blood and 7.4+/-2.32 to 10.0+/-3.27 in bone marrow. Pairwise comparison of the data did not reveal statistically significant differences between the control and mice with or without UWBR exposure groups (excluding positive controls).
Under the experimental conditions tested, there was no evidence for excess genotoxicity in peripheral blood or bone marrow cells of mice exposed to UWBR.
研究暴露于超宽带电磁辐射（UWBR）的小鼠外周血和骨髓细胞中的遗传损伤程度。
将CF-1雄性小鼠暴露于UWBR下15分钟，估计全身平均比吸收率为37 mW·kg⁻¹。研究还包括未处理的对照小鼠组和注射丝裂霉素C的阳性对照小鼠组。经过各种处理后，一半小鼠在18小时处死，另一半在24小时处死。检查外周血和骨髓涂片以确定遗传毒性程度，通过多色红细胞（PCE）中微核（MN）的存在来评估。
在18小时处死的小鼠的两个组织中，PCE的百分比和每2000个PCE的MN发生率与在24小时处死的小鼠中观察到的频率相似。对照小鼠与暴露或未暴露于UWBR的小鼠之间，PCE百分比没有显著差异；外周血中的组均值（±标准差）在3.1±0.14至3.2±0.23范围内，骨髓中的组均值在49.0±3.56至52.3±4.02范围内。对照小鼠以及暴露或未暴露于UWBR的小鼠中，每2000个PCE的MN平均发生率在外周血中为7.7±2.00至9.7±2.54，在骨髓中为7.4±2.32至10.0±3.27。数据的成对比较未显示对照小鼠与暴露或未暴露于UWBR的小鼠组（不包括阳性对照）之间存在统计学显著差异。
在测试的实验条件下，没有证据表明暴露于UWBR的小鼠外周血或骨髓细胞中存在过量的遗传毒性。
