Dey R D, Satterfield B, Altemus J B
Department of Anatomy, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown 26506, USA.
Anat Rec. 1999 Feb 1;254(2):166-72. doi: 10.1002/(SICI)1097-0185(19990201)254:2<166::AID-AR2>3.0.CO;2-E.
The neurochemical profiles of neurons in ferret tracheal ganglia has been characterized, but their projections to smooth muscle and epithelium in ferret trachea has not been examined. The purpose of this study is to determine the location of cell bodies that project VIP-, SP-, and NPY-containing fibers to the ferret tracheal smooth muscle and epithelium. Segments of ferret trachea were cultured for 0, 1, 3, or 7 days, some in the presence of 3 microm capsaicin. VIP, SP, or NPY nerve fiber density was measured using morphometric procedures. A retrograde tracer, rhodamine-labeled microspheres, identified neurons projecting to the epithelium. The density of SP fibers in the epithelium was reduced after culture, but VIP innervation was not different. In tracheal smooth muscle, the density of VIP- and SP-IR fibers was not different during the culture period, but NPY fiber density was reduced at all culture times. Capsaicin treatment did not affect nerve fiber density in the tracheal smooth muscle but produced a significant reduction in the density of epithelial VIP- and SP-IR nerve fibers after 1 day. Rhodamine-labeled microspheres were identified in VIP-containing nerve cell bodies of the ferret tracheal plexus. VIP innervation to the airway epithelium in ferret originates both from cell bodies in airway ganglia and cell bodies in sensory ganglia. The pathway from airway ganglia suggest the existence of a local reflex mechanisms initiated by epithelial irritation.
雪貂气管神经节中神经元的神经化学特征已得到描述，但它们向雪貂气管平滑肌和上皮的投射尚未得到研究。本研究的目的是确定向雪貂气管平滑肌和上皮投射含血管活性肠肽（VIP）、P物质（SP）和神经肽Y（NPY）纤维的细胞体的位置。将雪貂气管段培养0、1、3或7天，一些在3微摩尔辣椒素存在的情况下培养。使用形态计量学方法测量VIP、SP或NPY神经纤维密度。一种逆行示踪剂，罗丹明标记的微球，用于识别投射到上皮的神经元。培养后上皮中SP纤维的密度降低，但VIP神经支配没有差异。在气管平滑肌中，培养期间VIP和SP免疫反应性（IR）纤维的密度没有差异，但NPY纤维密度在所有培养时间均降低。辣椒素处理不影响气管平滑肌中的神经纤维密度，但1天后上皮VIP和SP-IR神经纤维的密度显著降低。在雪貂气管丛含VIP的神经细胞体中发现了罗丹明标记的微球。雪貂气道上皮的VIP神经支配既来自气道神经节中的细胞体，也来自感觉神经节中的细胞体。来自气道神经节的通路提示存在由上皮刺激引发的局部反射机制。
