Vilei E M, Nicolet J, Frey J
Institute for Veterinary Bacteriology, University of Berne, CH-3012 Berne, Switzerland.
J Bacteriol. 1999 Feb;181(4):1319-23. doi: 10.1128/JB.181.4.1319-1323.1999.
A new insertion sequence, IS1634, has been identified in Mycoplasma mycoides subsp. mycoides small-colony type (SC). IS1634 shows structural and functional similarities to IS1549 of Mycobacterium smegmatis and with it seems to form a new class or family of insertion sequences. IS1634 has a size of 1,872 bp, including two 13-bp terminal inverted repeats. It contains an open reading frame (ORF) encoding a product of 533 amino acids which shows similarity to the transposase of IS1549 and to a lesser extent to the transposases of IS elements of the IS4 family. IS1634 is present at about 30 copies in the genome of all 22 different field strains of M. mycoides subsp. mycoides SC tested. Characteristic of IS1634 are the long and variable-length direct repeats at the sites of insertion which were found to reach up to about 500 bp. IS1634 is specific to M. mycoides subsp. mycoides SC and is not present in any of the other members of the M. mycoides cluster. Neither was it found in other closely related Mycoplasma species of ruminants.
在蕈状支原体丝状亚种小菌落型（SC）中鉴定出一种新的插入序列IS1634。IS1634与耻垢分枝杆菌的IS1549在结构和功能上具有相似性，并且似乎与之形成了一个新的插入序列类别或家族。IS1634大小为1872 bp，包括两个13 bp的末端反向重复序列。它包含一个开放阅读框（ORF），编码一个533个氨基酸的产物，该产物与IS1549的转座酶相似，在较小程度上与IS4家族IS元件的转座酶相似。在所有测试的22种不同的蕈状支原体丝状亚种SC野外菌株的基因组中，IS1634约有30个拷贝。IS1634的特征是在插入位点存在长且长度可变的正向重复序列，发现其长度可达约500 bp。IS1634是蕈状支原体丝状亚种SC所特有的，在蕈状支原体簇的任何其他成员中均不存在。在其他密切相关的反刍动物支原体物种中也未发现它。
