Fujimoto T, Hata T, Itoyama T, Nakamura H, Tsukasaki K, Yamada Y, Ikeda S, Sadamori N, Tomonaga M
Department of Hematology, Nagasaki University School of Medicine, Japan.
Cancer Genet Cytogenet. 1999 Feb;109(1):1-13. doi: 10.1016/s0165-4608(98)00141-1.
To detect chromosomal abnormalities in prodromal phase of adult T-cell leukemia (ATL), we established a clonal culture method for human T-lymphotropic virus type I (HTLV-I) infected T-cells in methylcellulose containing recombinant human interleukin 2 (rhIL-2). We tried to analyze chromosomes of 187 colonies (4, 23, 69, 74, and 17, from HTLV-I-uninfected normal T-cells, HTLV-I-Infected normal T-cells, HTLV-I carriers, smoldering ATL, and chronic ATL, respectively), using chromosomal banding methods. In the prodromal group, 53% of colonies (84/160) (36/69, 37/74, 11/17 in HTLV-I carriers, smoldering ATLs, and chronic ATL, respectively) had chromosomal abnormal clones. In HTLV-I carriers, multiple clones with simple chromosomal abnormalities were observed. In more progressed chronic ATL, more complex chromosomal abnormalities were detected, and specific colonies were selected. Thus, colonies in the prodromal phase of ATL are characterized by cytogenetical clonal evolution and clonal changes.
为检测成人T细胞白血病（ATL）前驱期的染色体异常，我们建立了一种在含重组人白细胞介素2（rhIL-2）的甲基纤维素中对人I型嗜T淋巴细胞病毒（HTLV-I）感染的T细胞进行克隆培养的方法。我们尝试使用染色体显带方法分析187个集落（分别来自HTLV-I未感染的正常T细胞、HTLV-I感染的正常T细胞、HTLV-I携带者、冒烟型ATL和慢性ATL的4个、23个、69个、74个和17个）的染色体。在前驱组中，53%的集落（160个中的84个）（HTLV-I携带者、冒烟型ATL和慢性ATL中分别为36/69、37/74、11/17）有染色体异常克隆。在HTLV-I携带者中，观察到多个具有简单染色体异常的克隆。在进展更明显的慢性ATL中，检测到更复杂的染色体异常，并选择了特定的集落。因此，ATL前驱期的集落具有细胞遗传学克隆进化和克隆变化的特征。
