Johnson R E, Prakash S, Prakash L
Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, 6.104 Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061, USA.
Science. 1999 Feb 12;283(5404):1001-4. doi: 10.1126/science.283.5404.1001.
The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine-thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase eta.
酿酒酵母的RAD30基因是紫外线照射损伤DNA后进行无差错复制后修复所必需的。本文表明，RAD30编码一种DNA聚合酶，它能够高效复制通过胸腺嘧啶-胸腺嘧啶顺式-环丁烷二聚体，这种损伤通常会阻断DNA聚合酶。当与所有四种核苷酸在体外一起孵育时，Rad30在胸腺嘧啶-胸腺嘧啶二聚体相对位置掺入两个腺嘌呤。Rad30是被描述的第七种真核DNA聚合酶，因此被命名为DNA聚合酶η。
