Poljakova-Krusteva O, Donchev N, Gorchilova L, Krustev L
Z Parasitenkd. 1976 Oct 12;50(3):285-91. doi: 10.1007/BF02462973.
In the present paper the ultrastructure of the subcuticulare zone of the tegument of Fasciola hepatica and the distribution of thimidine-H3 for establishing the localization of DNA-synthesis in the tegument was studied. There were two types of subcuticular cells: 1) non-differentiated cells with presence of incorporated labelled thimidine in nuclei presented by DNA non-stable macromolecules, and 2) differentiated or definitive subcuticular cells without aggregates of silver grains. The results showed a high level of DNA replication leading to mitotic activity of the non-differentiated cells and absence of mitotic activity in the definitive cells. There was a morphological similarity between non-differentiated cells and the cells from the medular zone, supposing a relationship in their origin.
本文研究了肝片吸虫体表皮下区的超微结构以及利用胸腺嘧啶-H3分布来确定DNA合成在体表的定位。皮下细胞有两种类型：1）未分化细胞，其细胞核中存在掺入的标记胸腺嘧啶，由DNA不稳定大分子呈现；2）分化的或终末皮下细胞，无银颗粒聚集。结果表明，未分化细胞的DNA复制水平很高，导致有丝分裂活性，而终末细胞无有丝分裂活性。未分化细胞与髓质区细胞在形态上相似，推测它们在起源上有关系。
