McCutchan F E, Carr J K, Bajani M, Sanders-Buell E, Harry T O, Stoeckli T C, Robbins K E, Gashau W, Nasidi A, Janssens W, Kalish M L
The Henry M. Jackson Foundation, 1600 East Gude Drive, Rockville, Maryland, 20850, USA.
Virology. 1999 Feb 15;254(2):226-34. doi: 10.1006/viro.1998.9505.
Multiple human immunodeficiency virus type 1 (HIV-1) genetic subtypes, intersubtype recombinants, and group O have been found in west central Africa. In Nigeria, where HIV-1 prevalence is rising rapidly, characterization of HIV-1 strains has been limited. Each of three full-length genome sequences acquired to date shows evidence of recombination: two are largely subtype G with subtype A segments in the midgenome accessory region; the third, IbNG, is subtype G with the long terminal repeats and two segments of pol from subtype A. In this study, peripheral blood mononuclear cells obtained in 1994-1995 from 10 patients hospitalized in northeastern Nigeria were evaluated by sequencing of the complete envelope and, from 7 patients, a portion of gag. Four patients harbored subtype G viruses and six patients had recombinant viruses. Two had strains sharing the A/G recombinant structure of IbNG. Two had a previously undescribed recombinant, mostly subtype A, whose carboxyl-terminal gp41 could not be classified. An A/G recombinant different from IbNG but similar to CA1, a Cameroonian strain, was found in one patient. The remaining patient had a strain that was otherwise subtype G but shared an unclassified carboxyl-terminal gp41 segment with the CA1-like strains. Other subtypes and group O were not found.
在非洲中西部已发现多种1型人类免疫缺陷病毒（HIV-1）基因亚型、亚型间重组体和O组病毒。在HIV-1流行率迅速上升的尼日利亚，对HIV-1毒株的特征分析一直很有限。迄今为止获得的三个全长基因组序列均显示出重组迹象：两个主要是G亚型，在基因组中部辅助区域含有A亚型片段；第三个，即IbNG，是G亚型，其长末端重复序列和pol基因的两个片段来自A亚型。在本研究中，对1994年至1995年从尼日利亚东北部住院的10名患者获取的外周血单核细胞进行了全包膜测序评估，并对其中7名患者的部分gag基因进行了测序。4名患者携带G亚型病毒，6名患者携带重组病毒。两名患者的毒株具有与IbNG相同的A/G重组结构。两名患者有一种以前未描述的重组病毒，主要是A亚型，其羧基末端的gp41无法分类。在一名患者中发现了一种不同于IbNG但与喀麦隆毒株CA1相似的A/G重组体。其余一名患者的毒株在其他方面为G亚型，但与CA1样毒株共享一个未分类的羧基末端gp41片段。未发现其他亚型和O组病毒。
