小鼠γ-疱疹病毒68糖蛋白150可预防病毒诱导的单核细胞增多症：一种γ-疱疹病毒疫苗接种的模型系统。

Murine gamma-herpesvirus 68 glycoprotein 150 protects against virus-induced mononucleosis: a model system for gamma-herpesvirus vaccination.

作者信息

Stewart J P, Micali N, Usherwood E J, Bonina L, Nash A A

机构信息

Department of Veterinary Pathology, University of Edinburgh, UK.

出版信息

Vaccine. 1999 Jan;17(2):152-7. doi: 10.1016/s0264-410x(98)00190-x.

DOI:10.1016/s0264-410x(98)00190-x

PMID:9987149

Abstract

Murine gamma-herpesvirus 68 (MHV-68) is a model for the study of the pathogenesis of gamma-herpesviruses. Epstein-Barr virus (EBV) is a highly related gamma-herpesvirus that causes significant disease in humans. The major membrane antigen gp350 of EBV is a candidate vaccine antigen for protection against EBV-related disease. An MHV-68 glycoprotein, gp150, has significant homology to EBV gp350. We have therefore used the MHV-68 gp150 to model the potential efficacy of EBV gp350 in protecting from virus-associated disease. A recombinant vaccinia virus expressing MHV-68 gp150 was constructed. This recombinant vaccinia virus was used to infect mice via the subcutaneous route. This vaccination resulted in production of MHV-68-neutralising antibodies. Mice were then challenged intra-nasally with MHV-68. MHV-68-associated mononucleosis was virtually abrogated in immunised mice. However, mice did establish MHV-68 latency. The results suggest that gp350 may be effective as an immunogen to prevent EBV-associated infectious mononucleosis in humans that are EBV-seronegative.

摘要

小鼠γ-疱疹病毒68（MHV-68）是研究γ-疱疹病毒发病机制的模型。爱泼斯坦-巴尔病毒（EBV）是一种高度相关的γ-疱疹病毒，可导致人类严重疾病。EBV的主要膜抗原gp350是预防EBV相关疾病的候选疫苗抗原。一种MHV-68糖蛋白gp150与EBV gp350具有显著同源性。因此，我们利用MHV-68 gp150来模拟EBV gp350在预防病毒相关疾病方面的潜在功效。构建了一种表达MHV-68 gp150的重组痘苗病毒。这种重组痘苗病毒通过皮下途径感染小鼠。这种疫苗接种导致产生了MHV-68中和抗体。然后给小鼠鼻内接种MHV-68。免疫小鼠中与MHV-68相关的单核细胞增多症几乎被消除。然而，小鼠确实建立了MHV-68潜伏感染。结果表明，gp350作为一种免疫原可能有效预防EBV血清阴性的人类发生EBV相关的传染性单核细胞增多症。