Gassler N, Rastar F, Hentz M W
Institute for Anatomy and Cell Biology I, University of Heidelberg, Germany.
Histol Histopathol. 1999 Jan;14(1):81-7. doi: 10.14670/HH-14.81.
Transmyocardial revascularization (TMR) with CO2-laser equipment is an alternative approach in the treatment of patients with severe ischemic cardiac disease. Several studies concerning morphological features after TMR document a strong transmyocardial injury, but little is known about wound healing in laser-induced alterations of the cardiac skeleton and their putative role for angiogenesis and endothelialization. The present study was conducted to establish a useful immunohistochemical marker for detection of these laser-induced injuries and to analyze starting points of angiogenesis in human myocardium after TMR. Our data show that tenascin labeling is a useful immunohistochemical approach to detect laser-alterated segments of the cardiac skeleton as well as laser-induced fibrosis. Starting points of the angiogenetic process are seen throughout the margins of laser-induced lesions where myocardial capillaries are found. Disrupted vessels located within laser-alterated connective tissue septa are not major starting points for endothelialization of laser-induced lesions and for capillary sprouts. In comparison to laser-induced fibrosis, induction and promotion of angiogenesis by laser radiation is weak.
使用二氧化碳激光设备进行心肌血运重建术（TMR）是治疗严重缺血性心脏病患者的一种替代方法。几项关于TMR术后形态学特征的研究记录了严重的心肌损伤，但对于激光诱导的心脏支架改变后的伤口愈合及其在血管生成和内皮化中的假定作用知之甚少。本研究旨在建立一种有用的免疫组织化学标记物来检测这些激光诱导的损伤，并分析TMR术后人类心肌中血管生成的起始点。我们的数据表明，肌腱蛋白标记是一种有用的免疫组织化学方法，可检测心脏支架的激光改变节段以及激光诱导的纤维化。血管生成过程的起始点出现在激光诱导损伤的整个边缘，此处可见心肌毛细血管。位于激光改变的结缔组织间隔内的受损血管不是激光诱导损伤内皮化和毛细血管芽的主要起始点。与激光诱导的纤维化相比，激光辐射对血管生成的诱导和促进作用较弱。
