在使用改良培养基改善精子结合后，将受精失败的卵母细胞用于诊断性透明带结合目的。
Use of failed-fertilized oocytes for diagnostic zona binding purposes after sperm binding improvement with a modified medium.
作者信息
Henkel R, Müller C, Stalf T, Schill W B, Franken D R
机构信息
Center of Dermatology and Andrology, Justus Liebig University Giessen, Germany.
出版信息
J Assist Reprod Genet. 1999 Jan;16(1):24-9. doi: 10.1023/a:1022541511875.
PURPOSE
Because the availability of prophase oocytes for zona binding testing is limited, we compared sperm binding to the zona of failed-fertilized intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) oocytes after incubation in a standard IVF medium and a specially composed binding improvement medium.
METHODS
Semen samples from nine patients and nine fertile donors were separated in parallel by the standard swim-up method in both media. Subsequently, hemizona assays were performed with prophase, failed-fertilized ICSI and IVF oocytes.
RESULTS
Sperm separation resulted in a significantly higher sperm count (P < 0.01) and progressive motility (P = 0.018) in binding improvement medium. Moreover, spermatozoa coincubated with hemizonae (prophase, failed-fertilized ICSI and IVF oocytes) in binding improvement medium bound significantly more to hemizonae than in the controls (P < 0.01). However, the hemizona index did not differ.
CONCLUSIONS
Thus, the limited number of human zonae can be increased by the use of oocytes that failed to fertilize during ICSI or IVF. This will lead to a qualitative improvement of the diagnostic spectrum in male-factor infertility.
目的
由于用于透明带结合测试的前期卵母细胞数量有限，我们比较了在标准体外受精培养基和特制的结合改善培养基中孵育后，精子与胞浆内单精子注射（ICSI）和体外受精（IVF）失败的卵母细胞透明带的结合情况。
方法
对9例患者和9例有生育能力的供者的精液样本，在两种培养基中采用标准上浮法进行平行分离。随后，对前期、ICSI和IVF受精失败的卵母细胞进行半透明带试验。
结果
在结合改善培养基中进行精子分离，精子计数显著更高（P<0.01），进行性运动能力更强（P = 0.018）。此外，在结合改善培养基中与半透明带（前期、ICSI和IVF受精失败的卵母细胞）共同孵育的精子与半透明带的结合明显多于对照组（P<0.01）。然而，半透明带指数没有差异。
结论
因此，通过使用ICSI或IVF过程中受精失败的卵母细胞，可以增加人类透明带的数量。这将导致男性因素不孕症诊断范围的质量改善。
Zotero 插件