Hellmann D B, Pitt B
Am J Physiol. 1976 Nov;231(5 Pt. 1):1495-1500. doi: 10.1152/ajplegacy.1976.231.5.1495.
The effect of ethyl adenosine-5'-carboxylate hydrochloride (EA) was determined in 17 dogs by the radioactive microsphere technique. In both experimental and control animals, microspheres were injected 5 min before (preocclusion period), 60 min after (occlusion period), and 75 min after (saline or vasodilation period) ligation of the left anterior descending coronary artery (LAD). Ten seconds prior to the third microsphere injection, saline was administered to five control animals, and saline with 0.5 mg/kg of EA was given to 12 experimental animals. There was no significant change in myocardial blood flow after saline injection. After EA, mean arterial pressure fell 10% (P less than .001); myocardial blood flow increased 275% (P less than .001) in the nonischemic left circumflex artery (CIRC) area and increased 82% (P less than .01) in the occluded LAD area. In addition, those animals with extensive collateral development showed a significantly greater increase in collateral blood flow than those with minimal collateral development (P less than .05). Thus, EA may increase flow to ischemic areas of myocardium dependent upon the extent of preexistent collateral supply.
采用放射性微球技术在17只犬身上测定了盐酸5'-羧基腺苷乙酯（EA）的作用。在实验动物和对照动物中，均于左冠状动脉前降支（LAD）结扎前5分钟（闭塞前期）、结扎后60分钟（闭塞期）和结扎后75分钟（生理盐水或血管舒张期）注射微球。在第三次注射微球前10秒，给5只对照动物注射生理盐水，给12只实验动物注射含0.5mg/kg EA的生理盐水。注射生理盐水后心肌血流量无显著变化。注射EA后，平均动脉压下降10%（P<0.001）；非缺血性左旋支动脉（CIRC）区域的心肌血流量增加275%（P<0.001），闭塞的LAD区域的心肌血流量增加82%（P<0.01）。此外，侧支循环广泛发育的动物侧支血流量的增加明显大于侧支循环发育极少的动物（P<0.05）。因此，EA可能会根据预先存在的侧支供应程度增加流向心肌缺血区域的血流量。
