Nayak R K, Albert E N, Kassira W N
Am J Vet Res. 1976 Dec;37(12):1421-33.
Ultrastructural changes in the uterine tube (oviduct) of pregnant gilts have been investigated with special reference to the ciliated, secretory, and stromal cells. Tissue from the uterine tube ampulla and infundibulum was taken from 18 gilts at different stages of gestation (days 31, 36, 101, 102, 107, 110, and 112). Cilia were present throughout pregnancy, and deciliation was not apparent at any stage of gestation. The low epithelium of the uterine tube appeared similar to that of the luteal phase of the estrous cycle when corpora lutea were full grown. Prominent features at end of the gestation were numerous fibrous granules and basal bodies, indicating active formation of ciliary precursor organelles. Fibrogranular aggregates were also present in association with the basal bodies. In addition, numerous polyribosomes, mitochondria, and microtubules were encountered in the cytoplasm of ciliated cells at end of the gestation. The appearance of electron-opaque, fibrous granules during late pregnancy probably could be correlated with increasing endogenous levels of plasma estrogen. Intimate morphologic association between fibrous granules and basal bodies indicate that fibrous granules might provide precursor material for the development of cilia or rootlets. Characteristics ultrastructural changes observed in secretory cells during the estrous cycle were not discernible in secretory cells during pregnancy. The secretory cells appeared similar to those of the luteal phase of the estrous cycle. The apocrine secretory cells contained prominent, apical, cytoplasmic projections; pinching-off process of these protrusions was frequently observed during early and term gestation. Extruded nuclei along with other cytoplasmic organelles were also present, lying free in the tubal lumen. The endoplasmic reticulum was predominantly tubular in form. Synthesis, storage, and release of secretory granules were not apparent at early or late pregnancy. It is suggested that progesterone might have an inhibitory effect on the synthesis, storage, and release of secretory granules. Ultrastructural changes in stromal cells were not apparent at any stage of gestation. The stromal cells appeared similar to that of the luteal phase of the estrous cycle.
已对妊娠后备母猪输卵管的超微结构变化进行了研究，特别关注了纤毛细胞、分泌细胞和基质细胞。取自妊娠不同阶段（第31、36、101、102、107、110和112天）的18头后备母猪的输卵管壶腹部和漏斗部组织。整个妊娠期均有纤毛存在，在妊娠的任何阶段均未出现明显的纤毛脱失。当黄体充分发育时，输卵管的低柱状上皮与发情周期黄体期的上皮相似。妊娠末期的突出特征是大量纤维颗粒和基体，表明纤毛前体细胞器正在活跃形成。纤维颗粒聚集体也与基体相关存在。此外，在妊娠末期，纤毛细胞的细胞质中出现了大量多核糖体、线粒体和微管。妊娠后期出现的电子不透明纤维颗粒可能与血浆雌激素内源性水平升高有关。纤维颗粒与基体之间密切的形态学关联表明，纤维颗粒可能为纤毛或纤毛根部的发育提供前体物质。在发情周期中观察到的分泌细胞的特征性超微结构变化在妊娠期间的分泌细胞中无法辨别。分泌细胞与发情周期黄体期的分泌细胞相似。顶浆分泌细胞含有突出的顶端细胞质突起；在妊娠早期和足月时经常观察到这些突起的脱落过程。挤出的细胞核以及其他细胞质细胞器也存在，游离于输卵管腔内。内质网主要呈管状。在妊娠早期或晚期，分泌颗粒的合成、储存和释放均不明显。提示孕酮可能对分泌颗粒的合成、储存和释放具有抑制作用。在妊娠的任何阶段，基质细胞的超微结构变化均不明显。基质细胞与发情周期黄体期的基质细胞相似。
