Michelacci Y M, Dietrich C P
Biochim Biophys Acta. 1976 Dec 21;451(2):436-43. doi: 10.1016/0304-4165(76)90138-0.
The structures of chondroitin sulfate A from whale cartilage and chondroitin sulfate C from shark cartilage have been examined with the aid of the chondroitinases AC and C from Flavobacterium heparinum. The analyses of the products formed from the chondroitin sulfates by the action of the chondroitinases have shown that three types of oligosaccharides compose the structure of chondroitin sulfate A, namely, a dodeca-, hexa- and a tetra-saccharide, containing five, two and one 4-sulfated disaccharides per 6-sulfated disaccharide residue, respectively. The polymer contains an average of 3 mol of each oligosaccharide per mol of chondroitin sulfate A. Each mol of chondroitin sulfate C contains an average of 5 mol of 4-sulfated disaccharide units. A tetra-saccharide containing one 4-sulfated disaccharide and one 6-sulfated disaccharide C indicating that the 4-sulfated disaccharides are not linked together in one specific region but spaced in the molecule.
借助来自肝素黄杆菌的软骨素酶AC和C，对鲸软骨中的硫酸软骨素A和鲨软骨中的硫酸软骨素C的结构进行了研究。软骨素酶作用于硫酸软骨素形成的产物分析表明，三种类型的寡糖构成了硫酸软骨素A的结构，即一种十二糖、一种六糖和一种四糖，每一个6-硫酸化二糖残基分别含有五个、两个和一个4-硫酸化二糖。每摩尔硫酸软骨素A平均含有3摩尔每种寡糖。每摩尔硫酸软骨素C平均含有5摩尔4-硫酸化二糖单元。一种含有一个4-硫酸化二糖和一个6-硫酸化二糖C的四糖，表明4-硫酸化二糖并非在一个特定区域连接在一起，而是在分子中呈间隔分布。
