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酿酒酵母中同宗配合基因HMα和HMa的定位

Mapping of the homothallic genes, HM alpha and HMa, in Saccharomyces yeasts.

作者信息

Harashima S, Oshima Y

出版信息

Genetics. 1976 Nov;84(3):437-51. doi: 10.1093/genetics/84.3.437.

DOI:10.1093/genetics/84.3.437
PMID:1001877
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1213588/
Abstract

Two of the three homothallic genes, HM alpha and HMa, showed direct linkage to the mating-type locus at approximately 73 and 98 strans (57 and 65 centimorgans [cM], respectively, whereas, the other, HO, showed no linkage to 25 standard markers distributed over 17 chromosomes including the mating-type locus. To determine whether the HM alpha and HMa loci located on the left or right side of the mating-type locus, equations for three factor analysis of three linked genes were derived. Tetrad data were collected and were compared with expected values by chi 2 statistics. Calculations indicated that the HM alpha gene is probably located on the right arm at 95 strans (65 cM) from the centromere and the HMa locus at approximately 90 strans (64 cM) on the left arm of chromosome III.

摘要

三个同宗配合基因中的两个，即HMα和HMa，分别在约73和98个斯特恩斯（分别为57和65厘摩 [cM]）处与交配型位点直接连锁，而另一个基因HO与分布在包括交配型位点在内的17条染色体上的25个标准标记均无连锁关系。为了确定HMα和HMa位点位于交配型位点的左侧还是右侧，推导了三个连锁基因的三因子分析方程。收集了四分子数据，并通过卡方统计与预期值进行比较。计算表明，HMα基因可能位于右臂距着丝粒95个斯特恩斯（65 cM）处，而HMa位点位于第三条染色体左臂约90个斯特恩斯（64 cM）处。

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Genetics. 1967 Dec;57(4):875-85. doi: 10.1093/genetics/57.4.875.

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