Developmental Genetics Section, Gene Regulation and Chromosome Biology Laboratory, National Institutes of Health, National Cancer Institute, Frederick, MD 21702-1021, USA.
Genetics. 2010 Oct;186(2):443-9. doi: 10.1534/genetics.110.122531.
It has been 33 years since I first presented results of genetic experiments that established the gene transposition model as the mechanism of mating-type switching in the budding yeast Saccharomyces cerevisiae at the Cold Spring Harbor Laboratory (CSHL) Yeast Genetics meeting in August 1977. Over two decades ago the Genetics Perspectives editors solicited a perspective on my participation in the studies that deciphered the mechanism of mating-type switching and revealed the phenomenon of gene silencing in yeast. Although flattered at the time, I thought that preparation of such an article called for a more seasoned researcher who had benefitted from seeing his contributions stand the test of time. Now realizing that our discovery of the transposition of a mutation from the HMα locus into the MAT (mating type) locus has provided the genetic evidence that established the gene transposition model, and having witnessed our conclusions confirmed by subsequent molecular studies, I decided that perhaps this is a good time to recount the chronology of events as they unfolded for me decades ago.
自 1977 年 8 月我在冷泉港实验室（CSHL）酵母遗传学会议上首次展示遗传实验结果，确立了酿酒酵母交配型转换的基因转位模型以来，已经过去了 33 年。二十多年前，《遗传学透视》（Genetics Perspectives）的编辑们征求了我对参与揭示交配型转换机制和酵母基因沉默现象的研究的看法。当时虽然感到受宠若惊，但我认为撰写这样一篇文章需要一位更有经验的研究人员，他从自己的贡献经得起时间考验中受益。现在我意识到，我们发现 HMα 基因座的突变易位到 MAT（交配型）基因座提供了遗传证据，确立了基因转位模型，并且我们的结论已经被随后的分子研究证实，我决定，也许现在是时候回顾一下几十年前我所经历的事件的时间顺序了。
