母亲摄入的牛乳β-乳球蛋白在人乳中不存在。酶联免疫吸附测定（ELISA）测量结果不可靠。
Absence in human milk of bovine beta-lactoglobulin ingested by the mother. Unreliability of ELISA measurements.
作者信息
Bertino E, Coscia A, Costa S, Farinasso D, Prandi G, Fabris C, Cavaletto M, Giuffrida M G, Conti A
机构信息
Cattedra di Neonatologia, Università di Torino, Italy.
出版信息
Acta Biomed Ateneo Parmense. 1997;68 Suppl 1:15-9.
OBJECTIVES
To analyze the presence of bovine beta-LG in breast milk.
METHODS
Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of beta-LG immuno-like proteins (beta-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing.
RESULTS
beta-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, beta-casein and alpha-lactalbumin, were identified as cross-reacting antigens.
CONCLUSIONS
False-positive results in ELISA determinations of bovine beta-LG in human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.
目的
分析母乳中牛β-乳球蛋白的存在情况。
方法
检测了14名健康、非特应性且食用不同牛奶含量饮食的女性的母乳样本。采用酶联免疫吸附测定法（ELISA）测定β-乳球蛋白免疫样蛋白（β-LGIP）的总浓度。通过N端测序鉴定抗原。
结果
不同饮食的受试者母乳中β-LGIP反应性无显著差异。人乳铁蛋白、β-酪蛋白和α-乳白蛋白被鉴定为交叉反应抗原。
结论
母乳中牛β-乳球蛋白ELISA测定中的假阳性结果可能是由于多克隆抗体与不同蛋白质抗原之间的交叉反应所致。
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