为什么杀虫剂靶标基因中与抗性相关的突变如此之少？
Why are there so few resistance-associated mutations in insecticide target genes?
作者信息
ffrench-Constant R H, Pittendrigh B, Vaughan A, Anthony N
机构信息
Department of Entomology, University of Wisconsin-Madison 53706, USA.
出版信息
Philos Trans R Soc Lond B Biol Sci. 1998 Oct 29;353(1376):1685-93. doi: 10.1098/rstb.1998.0319.
Abstract
The genes encoding the three major targets of conventional insecticides are: Rdl, which encodes a gamma-aminobutyric acid receptor subunit (RDL); para, which encodes a voltage-gated sodium channel (PARA); and Ace, which encodes insect acetylcholinesterase (AChE). Interestingly, despite the complexity of the encoded receptors or enzymes, very few amino acid residues are replaced in different resistant insects: one within RDL, two within PARA and three or more within AChE. Here we examine the possible reasons underlying this extreme conservation by looking at the aspects of receptor and/or enzyme function that may constrain replacements to such a limited number of residues.
摘要
编码传统杀虫剂三大主要作用靶标的基因分别是
Rdl，编码γ-氨基丁酸受体亚基（RDL）；para，编码电压门控钠通道（PARA）；以及Ace，编码昆虫乙酰胆碱酯酶（AChE）。有趣的是，尽管所编码的受体或酶结构复杂，但在不同的抗性昆虫中只有极少的氨基酸残基发生了替换：RDL中有一个，PARA中有两个，AChE中有三个或更多。在此，我们通过研究受体和/或酶功能中可能将替换限制在如此少量残基的方面，来探究这种极端保守性背后的潜在原因。
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