Guo W, Roth D, Walch-Solimena C, Novick P
Department of Cell Biology, Yale University School of Medicine, PO Box 208002, New Haven, CT 06520-8002, USA.
EMBO J. 1999 Feb 15;18(4):1071-80. doi: 10.1093/emboj/18.4.1071.
Polarized secretion requires proper targeting of secretory vesicles to specific sites on the plasma membrane. Here we report that the exocyst complex plays a key role in vesicle targeting. Sec15p, an exocyst component, can associate with secretory vesicles and interact specifically with the rab GTPase, Sec4p, in its GTP-bound form. A chain of protein-protein interactions leads from Sec4p and Sec15p on the vesicle, through various subunits of the exocyst, to Sec3p, which marks the sites of exocytosis on the plasma membrane. Sec4p may control the assembly of the exocyst. The exocyst may therefore function as a rab effector system for targeted secretion.
极化分泌需要将分泌囊泡正确靶向到质膜上的特定部位。在此我们报告，外排体复合物在囊泡靶向中起关键作用。外排体组分Sec15p可与分泌囊泡结合，并以其GTP结合形式与Rab GTP酶Sec4p特异性相互作用。一系列蛋白质-蛋白质相互作用从囊泡上的Sec4p和Sec15p开始，通过外排体的各种亚基，延伸至Sec3p，Sec3p标记了质膜上的胞吐作用位点。Sec4p可能控制外排体的组装。因此，外排体可能作为一种用于靶向分泌的Rab效应系统发挥作用。
