Echard A, Jollivet F, Martinez O, Lacapère J J, Rousselet A, Janoueix-Lerosey I, Goud B
Unité Mixte de Recherche CNRS 144 et 168, Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
Science. 1998 Jan 23;279(5350):580-5. doi: 10.1126/science.279.5350.580.
Rab guanosine triphosphatases regulate vesicular transport and membrane traffic within eukaryotic cells. Here, a kinesin-like protein that interacts with guanosine triphosphate (GTP)-bound forms of Rab6 was identified. This protein, termed Rabkinesin-6, was localized to the Golgi apparatus and shown to play a role in the dynamics of this organelle. The carboxyl-terminal domain of Rabkinesin-6, which contains the Rab6-interacting domain, inhibited the effects of Rab6-GTP on intracellular transport. Thus, a molecular motor is a potential effector of a Rab protein, and coordinated action between members of these two families of proteins could control membrane dynamics and directional vesicular traffic.
Rab鸟苷三磷酸酶调节真核细胞内的囊泡运输和膜转运。在此，鉴定出一种与鸟苷三磷酸（GTP）结合形式的Rab6相互作用的驱动蛋白样蛋白。这种蛋白称为Rab驱动蛋白-6，定位于高尔基体，并显示在该细胞器的动态变化中起作用。Rab驱动蛋白-6的羧基末端结构域包含与Rab6相互作用的结构域，可抑制Rab6-GTP对细胞内运输的影响。因此，分子马达是Rab蛋白的潜在效应物，这两类蛋白成员之间的协同作用可控制膜动态变化和定向囊泡运输。
