Stoltenburg R, Lösche O, Klappach G, Kunze G
Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, D-06466 Gatersleben, Germany.
Curr Genet. 1999 Feb;35(1):8-13. doi: 10.1007/s002940050426.
The yeast replication factor C (RF-C) is a multi-polypeptide complex with five sub-units involved in chromosomal DNA replication. This factor, encoded by five genes, is well characterized for the yeast Saccharomyces cerevisiae. However, RF-C components from other yeast species have not been analysed yet. Here we report the cloning and characterization of ARFC3 from the dimorphic and osmo-tolerant yeast Arxula adeninivorans. This gene encodes one subunit of the RF-C complex. It is localized on chromosome 1 of the four Arxula chromosomes and comprises a coding region of 1014 bp, which corresponds to 338 amino acids. Two introns are contained within this gene. The ARFC3 transcript level is influenced by both salt and temperature. The latter also influences the morphological state (budding cells, mycelium). High salt concentration and high temperature result in a rapid decrease of the ARFC3 mRNA.
酵母复制因子C（RF-C）是一种具有五个亚基的多聚体复合物，参与染色体DNA复制。该因子由五个基因编码，在酿酒酵母中已得到充分表征。然而，其他酵母物种的RF-C组分尚未进行分析。在此，我们报告了来自二态且耐渗透压酵母嗜腺嘌呤阿苏菌的ARFC3的克隆与表征。该基因编码RF-C复合物的一个亚基。它定位于阿苏菌四条染色体中的第1号染色体上，包含一个1014 bp的编码区，对应338个氨基酸。该基因含有两个内含子。ARFC3转录水平受盐和温度的影响。温度还影响形态状态（芽殖细胞、菌丝体）。高盐浓度和高温导致ARFC3 mRNA迅速减少。
