Chand-Goyal T, Eckert J W, Droby S, Glickmann E, Atkinson K
Department of Plant Pathology, University of California, Riverside, CA 92521, USA.
Curr Genet. 1999 Feb;35(1):51-7. doi: 10.1007/s002940050432.
Histidine auxotrophs of wild-type strain I-182 of Candida oleophila, produced using ethyl methanesulfonate, were transformed with plasmids containing the HIS3, HIS4 and HIS5 genes of Saccharomyces cerevisiae. Histidine auxotrophy was complemented by the HIS5 gene of S. cerevisiae. Stability of the transformants under non-selective conditions and DNA gel-blot analysis suggested that the transforming DNA had integrated into the C. oleophila genome. There were no detectable physiological differences between the wild-type and the transformants. The biological control ability of C. oleophila was not affected by the transformation. A genetically marked transformant (with a beta-glucuronidase gene) colonized wounds on oranges, and its population increased under field conditions. The identity of the genetically marked transformant was established by PCR-amplification of a portion of the beta-glucuronidase gene.
利用甲磺酸乙酯制备的嗜油酸假丝酵母野生型菌株I-182的组氨酸营养缺陷型，用含有酿酒酵母HIS3、HIS4和HIS5基因的质粒进行转化。酿酒酵母的HIS5基因补充了组氨酸营养缺陷。在非选择性条件下转化体的稳定性和DNA凝胶印迹分析表明，转化DNA已整合到嗜油酸假丝酵母基因组中。野生型和转化体之间没有可检测到的生理差异。嗜油酸假丝酵母的生物防治能力不受转化的影响。一个带有β-葡萄糖醛酸酶基因的遗传标记转化体在橙子伤口上定殖，其种群在田间条件下增加。通过对β-葡萄糖醛酸酶基因的一部分进行PCR扩增，确定了遗传标记转化体的身份。
