用含有酿酒酵母ARG4 DNA的质粒对麦芽糖假丝酵母和季也蒙毕赤酵母进行转化。
Transformation of Candida maltosa and Pichia guilliermondii by a plasmid containing Saccharomyces cerevisiae ARG4 DNA.
作者信息
Kunze G, Petzoldt C, Bode R, Samsonova I, Hecker M, Birnbaum D
机构信息
Sektion Biologie Ernst-Moritz-Arndt-Universität, Greifswald, German Democratic Republic.
出版信息
Curr Genet. 1985;9(3):205-9. doi: 10.1007/BF00420313.
Saccharomyces cerevisiae, Candida maltosa and Pichia guilliermondii have been transformed by the plasmid pYe(ARG4)411, which contains the S. cerevisiae ARG4 gene inserted into pBR322. In all transformants argininosuccinate lyase as well as beta-lactamase were detected. The ARG+ phenotype of transformants is mitotically unstable. Closed circular pYe(ARG4)411 DNA was detected in transformant DNA preparations by hybridization to pBR322 DNA and by transformation of E. coli to ampicillin resistance.
酿酒酵母、麦芽糖假丝酵母和季也蒙毕赤酵母已被质粒pYe(ARG4)411转化，该质粒含有插入pBR322的酿酒酵母ARG4基因。在所有转化体中均检测到精氨琥珀酸裂解酶和β-内酰胺酶。转化体的ARG+表型在有丝分裂过程中不稳定。通过与pBR322 DNA杂交以及将大肠杆菌转化为氨苄青霉素抗性，在转化体DNA制备物中检测到闭环pYe(ARG4)411 DNA。
Zotero 插件