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用含有酿酒酵母ARG4 DNA的质粒对麦芽糖假丝酵母和季也蒙毕赤酵母进行转化。

Transformation of Candida maltosa and Pichia guilliermondii by a plasmid containing Saccharomyces cerevisiae ARG4 DNA.

作者信息

Kunze G, Petzoldt C, Bode R, Samsonova I, Hecker M, Birnbaum D

机构信息

Sektion Biologie Ernst-Moritz-Arndt-Universität, Greifswald, German Democratic Republic.

出版信息

Curr Genet. 1985;9(3):205-9. doi: 10.1007/BF00420313.

DOI:10.1007/BF00420313
PMID:3916721
Abstract

Saccharomyces cerevisiae, Candida maltosa and Pichia guilliermondii have been transformed by the plasmid pYe(ARG4)411, which contains the S. cerevisiae ARG4 gene inserted into pBR322. In all transformants argininosuccinate lyase as well as beta-lactamase were detected. The ARG+ phenotype of transformants is mitotically unstable. Closed circular pYe(ARG4)411 DNA was detected in transformant DNA preparations by hybridization to pBR322 DNA and by transformation of E. coli to ampicillin resistance.

摘要

酿酒酵母、麦芽糖假丝酵母和季也蒙毕赤酵母已被质粒pYe(ARG4)411转化，该质粒含有插入pBR322的酿酒酵母ARG4基因。在所有转化体中均检测到精氨琥珀酸裂解酶和β-内酰胺酶。转化体的ARG+表型在有丝分裂过程中不稳定。通过与pBR322 DNA杂交以及将大肠杆菌转化为氨苄青霉素抗性，在转化体DNA制备物中检测到闭环pYe(ARG4)411 DNA。

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Transformation of Candida maltosa and Pichia guilliermondii by a plasmid containing Saccharomyces cerevisiae ARG4 DNA.用含有酿酒酵母ARG4 DNA的质粒对麦芽糖假丝酵母和季也蒙毕赤酵母进行转化。
Curr Genet. 1985;9(3):205-9. doi: 10.1007/BF00420313.
2
High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene.通过含有克隆的酵母ARG4基因的质粒对酵母进行高频转化。
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Evidence for autonomous replication and stabilization of recombinant plasmids in the transformants of yeast Hansenula polymorpha.多形汉逊酵母转化体中重组质粒自主复制和稳定的证据。
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Construction of a host-vector system in Candida maltosa by using an ARS site isolated from its genome.通过使用从麦芽糖假丝酵母基因组中分离出的自主复制序列（ARS）位点构建麦芽糖假丝酵母宿主-载体系统。
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Pichia pastoris as a host system for transformations.毕赤酵母作为转化的宿主系统。
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["Illegal" transformation of red adenine-dependent mutants of the yeast Pichia pinus by the pYE(ADE2)2 plasmid].["酵母毕赤酵母红色腺嘌呤依赖型突变体通过pYE(ADE2)2质粒进行的“非法”转化]
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Candida guilliermondii: biotechnological applications, perspectives for biological control, emerging clinical importance and recent advances in genetics.季也蒙假丝酵母：生物技术应用、生物防治的展望、新兴临床重要性和遗传研究的最新进展。
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The gene for the small ribosomal RNA on yeast mitochondrial DNA: Physical map, direction of transcription and absence of an intervening sequence.酵母线粒体 DNA 上小核糖体 RNA 基因：物理图谱、转录方向和无内含子。
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Construction of a host-vector system in Candida maltosa by using an ARS site isolated from its genome.通过使用从麦芽糖假丝酵母基因组中分离出的自主复制序列（ARS）位点构建麦芽糖假丝酵母宿主-载体系统。
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Transformation of Ustilago maydis by a plasmid containing yeast 2-micron DNA.玉米黑粉菌的质体转化：含酵母 2-μm 质粒 DNA。
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High-frequency transformation of the fission yeast Schizosaccharomyces pombe.高频转化裂殖酵母酿酒酵母。
Nature. 1981 Mar 12;290(5802):140-2. doi: 10.1038/290140a0.
6
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Cloning of a LEU gene and an ARS site of Candida maltosa.麦芽糖假丝酵母亮氨酸基因和自主复制序列位点的克隆
Gene. 1983 Oct;24(2-3):157-62. doi: 10.1016/0378-1119(83)90075-6.
8
The presence of a defective LEU2 gene on 2 mu DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number.酿酒酵母2微米DNA重组质粒上存在有缺陷的LEU2基因，这导致了质粒的消除和高拷贝数。
J Bacteriol. 1983 Nov;156(2):625-35. doi: 10.1128/jb.156.2.625-635.1983.
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Delta sequences and double symmetry in a yeast chromosomal replicator region.酵母染色体复制子区域中的δ序列和双重对称性
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
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